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Product Info Summary
| SKU: | M01465-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-IP3 Receptor Rabbit Monoclonal Antibody
SKU/Catalog Number
M01465-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-IP3 Receptor Rabbit Monoclonal Antibody catalog # M01465-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IP3 Receptor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01465-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17I50
Isotype
IgG
Immunogen
A synthesized peptide derived from human IP3 Receptor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01465-1 is reactive to ITPR1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
314 kDa
Calculated molecular weight
56782 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01465-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:500-1:1000
IP 1:50
Positive Control
WB: human SH-SY5Y whole cell, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IP3 Receptor using anti-IP3 Receptor antibody (M01465-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP3 Receptor antigen affinity purified monoclonal antibody (Catalog # M01465-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP3 Receptor at approximately 314 kDa. The expected band size for IP3 Receptor is at 314 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ITPR1 (Source: Uniprot.org, NCBI)
Gene Name
ITPR1
Full Name
Inositol 1,4,5-trisphosphate receptor type 1
Weight
56782 MW
Superfamily
InsP3 receptor family
Alternative Names
Protein disulfide-isomerase A3;5.3.4.1;58 kDa glucose-regulated protein;58 kDa microsomal protein;p58;Disulfide isomerase ER-60;Endoplasmic reticulum resident protein 57;ER protein 57;ERp57;Endoplasmic reticulum resident protein 60;ER protein 60;ERp60;PDIA3;ERP57, ERP60, GRP58; ITPR1 ACV, CLA4, INSP3R1, IP3R, IP3R1, PPP1R94, SCA15, SCA16, SCA29 inositol 1,4,5-trisphosphate receptor type 1 inositol 1,4,5-trisphosphate receptor type 1|IP3 receptor|IP3R 1|inositol 1,4,5-triphosphate receptor, type 1|protein phosphatase 1, regulatory subunit 94|type 1 InsP3 receptor|type 1 inositol 1,4,5-trisphosphate receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITPR1, check out the ITPR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITPR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IP3 Receptor Rabbit Monoclonal Antibody (M01465-1)
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