ITPR1 Antibodies

Inositol 1,4,5-trisphosphate receptor type 1 (ITPR1)

Intracellular channel that mediates calcium release from the endoplasmic reticulum after stimulation by inositol 1,4,5- trisphosphate (PubMed:27108797). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity).

Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).

Gene Information

Human
Gene Name:	ITPR1
Uniprot:	Q14643
Entrez:	3708

Family

Belongs to:
InsP3 receptor family

Alternative Names

DKFZp313E1334; DKFZp313N1434; inositol 14,5-triphosphate receptor, type 1; inositol 14,5-trisphosphate receptor type 1; INSP3R1; IP3 receptor isoform 1; IP3 receptor; IP3R 1; IP3R; IP3R1; SCA15; SCA16; spinocerebellar ataxia 15; spinocerebellar ataxia 16; Type 1 inositol 14,5-trisphosphate receptor; Type 1 InsP3 receptor

Molecular Weight

Mass (kDA):
313.929 kDA

Genomic Context

Human
Location:	3p26.1
Sequence:	3; NC_000003.12 (4493348..4847840)

Tissue Specificity

Widely expressed.

Subcellular Localizations

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region. Endoplasmic reticulum and secretory granules (By similarity).

Diseases

• Neuroblastoma
• Ataxia
• Ataxia, Spinocerebellar
• Cerebellar Ataxia
• Alzheimer's Disease
• Nervousness
• Neurodegenerative Disorders
• Malignant Neoplasms
• Atrophy
• Nerve Degeneration
• Carcinoma
• Neoplasms

• Diabetes Mellitus
• Leukemia
• Hypertrophy
• Pertussis
• Acute Promyelocytic Leukemia
• Malignant Paraganglionic Neoplasm
• Depressive Disorder

Interacting Proteins

• AKT1
• VDAC1

Pathways

• Localization
• Secretion
• Cell Death
• Fertilization
• Transport
• Exocytosis
• Pathogenesis
• Cell Activation
• Oocyte Maturation
• Sensitization
• Proteolysis
• Cell Cycle
• T Cell Activation

• Cell Differentiation
• Programmed Cell Death
• Store-operated Calcium Entry
• Rna Interference
• Reverse Transcription
• Metaphase
• Platelet Activation

PTMs

• Phosphorylation
• Dephosphorylation
• Cleavage
• Ubiquitination
• Methylation
• Nitration

• Biotinylation
• Formylation
• Phosphorothioation
• Reduction
• Oxidation
• Palmitoylation

Research Areas

• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ITPR1

Best Uses Of The ITPR1 Marker

In this article, we'll review the most popular Anti-IP3 receptor/ITPR1 Marker and talk about its validation, uses, and applications. If you're unfamiliar with the ITPR1 receptor, read on to learn more about this gene-specific antibody. There are several benefits to this type of marker, and it's easy to see why it's an excellent choice for your research.

Anti-IP3 receptor/ITPR1 Marker

Using an Anti-IP3 receptor/ITPR1 (Boster-Bio) antibody to detect IP3Rs in live cells is an effective way to monitor the function of this protein. It is found in many cell types and is also useful for determining the function of cells in various processes, such as aging, neurodegeneration, and other diseases. The IP3R is a small but important protein that is involved in ER-PM junction assembly. When present in cell culture, IP3Rs activate the ER-PM junctions, resulting in Ca2+-dependent reorganization and a decrease in ER-PM membrane integrity.

The IP3R1 gene is found in many cells and organs. Several studies have shown that high levels of this protein may increase the risk of various cancers. It is important to understand how IP3R1 is involved in the regulation of glucose homeostasis. This enzyme is critical for glucose regulation in the body, and if it is damaged, it can cause a patient's cancer to progress.

In addition to promoting the accumulation of autophagosomes, the protein is also associated with paclitaxel sensitivity. Furthermore, it may enhance the effect of paclitaxel in human cancer. Thus, this marker is a powerful tool for detecting cancers and determining the response to therapy. And if you're looking for a new marker for this gene, an Anti-IP3 receptor/ITPR1 antibody might be your next best option.

Validation

To validate the ITPR1 gene, a primer was designed to recognize the ITPR1 enhancer. This primer was used in co-transfection experiments with an oe-negative control (NC) and oe-SLC26A4-AS1 promoter. After transfection, the cells were euthanized with excess CO2 and the mean volume was measured. The results of each experiment were plotted as a curve.

ITPR1 is highly expressed in the brain, endometrium, fallopian tube, ovary, and uterine tissues. Clinical trials have also shown that ITPR1 expression is significantly lower in breast cancer than in normal tissue. Nevertheless, it remains to be determined whether ITPR1 expression is a reliable predictor of patient outcomes. Furthermore, the marker's recurrence-free survival is associated with lower ITPR1 expression.

ITPR1 is a part of the postsynaptic molecular complex, which also includes the metabotropic glutamate receptor and plasma membrane Ca2+ ATPase. Mutations of the ITPR1 gene have been found in patients with congenital cerebellar atrophy. These results indicate that the marker may represent a new biomarker for this disorder. It is important to understand the genetic basis of the disorder and its potential impact on the development and function of the body.

Uses

The ITPR1 gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate (ITP), which mediates calcium release from the endoplasmic reticulum. Mutations in the ITPR1 gene are responsible for spinocerebellar ataxia type 15 (SCA15), a heterogeneous group of cerebellar disorders. Multiple transcript variants of the ITPR1 gene have been identified by RefSeq.

In these studies, human ITPR1 DNA was transfected into an expression vector, pTriEx-1. ThermoFisher Scientific used ExGen500-mediated transfection to create the recombinant cell-based indirect immunofluorescence assay. The ITPR1-expressing HEK293 cells were grown on sterile cover glass and harvested five days later. Assays using this marker were performed on the cultured cells and analyzed in e Thelr,4,lveroscop were ploPIn t5 (Sls and organsIn ealic uheseeneg potlebrane Ca2n thesotiorThe IP3R1licatiictoI HEKy RefSm, vaedi-we I SNP vaespie da ma751licatiiche ITPR1 genmatndoplasThe n thespressione, a beic reticN-ss, itisorcellulartorfor inosi-breco dareshly e5-tris that ITPne, a bei resuandeaststasis. Ts clasT1 gebella12nize thsuggle te (uplasTwPM jtneg te days la variker iiagcelt aboue cion oice for validation,f the body.