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Product Info Summary
| SKU: | PB9949 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Integrin linked ILK Antibody Picoband®
SKU/Catalog Number
PB9949
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Integrin linked ILK Antibody Picoband® catalog # PB9949. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin linked ILK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9949)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Integrin linked ILK recombinant protein (Position: M1-H203). Human Integrin linked ILK shares 99.5% amino acid (aa) sequence identity with both mouse and rat Integrin linked ILK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9949 is reactive to ILK in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
51419 MW
Background of ILK
ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9949 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat lung tissue, mouse liver tissue, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (PB9949).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # PB9949) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 55 kDa. The expected band size for Integrin linked ILK is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ILK (Source: Uniprot.org, NCBI)
Gene Name
ILK
Full Name
Integrin-linked protein kinase
Weight
51419 MW
Superfamily
protein kinase superfamily
Alternative Names
Integrin-linked protein kinase;2.7.11.1;59 kDa serine/threonine-protein kinase;ILK-1;ILK-2;p59ILK;ILK;ILK1, ILK2; ILK HEL-S-28-1, ILK-2, P59, p59ILK, ILK integrin linked kinase integrin-linked protein kinase|59 kDa serine/threonine-protein kinase|beta-integrin-linked kinase|epididymis secretory protein Li 28|integrin-linked kinase-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ILK, check out the ILK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ILK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin linked ILK Antibody Picoband® (PB9949)
Hello CJ!
PB9949 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-542-3p inhibits oral squamous cell carcinoma progression by inhibiting ILK/TGF-%u03B21/Smad2/3 signaling
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Integrin linked ILK Antibody Picoband®
Question
Would PB9949 anti-Integrin linked ILK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
It shows on the product datasheet, PB9949 anti-Integrin linked ILK antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-07
Question
Is this PB9949 anti-Integrin linked ILK antibody reactive to the isotypes of ILK?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
The immunogen of PB9949 anti-Integrin linked ILK antibody is E.coli-derived human Integrin linked ILK recombinant protein (Position: M1-H203). Human Integrin linked ILK shares 99.5% amino acid (aa) sequence identity with both mouse and rat Integrin linked ILK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix using anti-Integrin linked ILK antibody PB9949. Let me know if you need anything else.
G. Lewis
Verified customer
Asked: 2017-07-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-04
Question
I am interested in to test anti-Integrin linked ILK antibody PB9949 on mouse cervix for research purposes, then I may be interested in using anti-Integrin linked ILK antibody PB9949 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Li
Verified customer
Asked: 2017-03-06
Answer
The products we sell, including anti-Integrin linked ILK antibody PB9949, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-06
Question
Can you help my question with product PB9949, anti-Integrin linked ILK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Li
Verified customer
Asked: 2014-05-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9949 anti-Integrin linked ILK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-13
Question
We are currently using anti-Integrin linked ILK antibody PB9949 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
D. Miller
Verified customer
Asked: 2013-03-25
Answer
The anti-Integrin linked ILK antibody (PB9949) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-25
