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Product Info Summary
| SKU: | M01015 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Integrin beta 4 ITGB4 Rabbit Monoclonal Antibody
View all Integrin beta 4/CD104 Antibodies
SKU/Catalog Number
M01015
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin beta 4 ITGB4 Rabbit Monoclonal Antibody catalog # M01015. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin beta 4 ITGB4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01015)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAFE-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin beta 4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01015 is reactive to ITGB4 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
240 kDa
Calculated molecular weight
202167 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01015 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: SW480 cell, Mouse spleen, human PC-3 whole cell, human Caco-2 whole cell, human Hela whole cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ITGB4 using anti-ITGB4 antibody (M01015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB4 antigen affinity purified monoclonal antibody (Catalog # M01015) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB4 at approximately 240 kDa. The expected band size for ITGB4 is at 202 kDa.
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Western blot analysis of Integrin beta 4 expression in (1) SW480 cell lysate; (2) Mouse spleen lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Integrin beta 4 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ITGB4 (Source: Uniprot.org, NCBI)
Gene Name
ITGB4
Full Name
Integrin beta-4
Weight
202167 MW
Superfamily
integrin beta chain family
Alternative Names
Integrin beta-4;GP150;CD104;ITGB4; ITGB4 CD104, GP150 integrin subunit beta 4 integrin beta-4|CD104
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGB4, check out the ITGB4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin beta 4 ITGB4 Rabbit Monoclonal Antibody (M01015)
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