Anti-Phospho-Insulin Receptor (Thr1160) INSR Antibody

Product Name

Anti-Phospho-Insulin Receptor (Thr1160) INSR Antibody

View all INSR Antibodies

SKU/Catalog Number

P00447

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Insulin Receptor (Thr1160) INSR Antibody (Catalog # P00447). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Insulin Receptor (Thr1160) INSR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00447)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1160 of human insulin receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00447 is reactive to INSR in Human

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

156333 MW

Background of INSR

The insulin receptor (IR) is a well-studied receptor tyrosine kinase composed of two α subunits, responsible for the extracellular insulin binding site, and two β subunits, responsible for intracellular protein kinase activity (Endemann et al., 1990, Chiu et al., 2010). The binding of insulin to the α subunits activates the intrinsic kinase activity located in the β subunits and subsequently initiates a cascade of phosphorylation events causing major conformational changes in the activation loop of the kinase domain, which lead to different biological functions (Chiu et al., 2010). It has been hypothesized that Thr-1160 phosphorylation affects or is affected by Tyr-1158/62/63 phosphorylation and that the conformation of Thr-1160 and pThr-1160 is likely to be affected by the phosphorylation status of the surrounding tyrosines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P00447 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For INSR (Source: Uniprot.org, NCBI)

Alternative Names

CD220 antibody, HHF5 antibody, human insulin receptor antibody, Insr antibody, INSR_HUMAN antibody, Insulin receptor subunit beta antibody, IR 1 antibody, IR antibody, IR-1 antibody, IR1 antibody, INSR CD220, HHF5 insulin receptor insulin receptor|IR

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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