Insulin receptor Antibodies

Insulin receptor (INSR)

Receptor tyrosine kinase which mediates the pleiotropic action of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates.

Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src- homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the regeneration of two primary signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation.

Gene Information

Human
Gene Name:	INSR
Uniprot:	P06213
Entrez:	3643

Family

Belongs to:
protein kinase superfamily

Alternative Names

CD220 antigen; CD220; EC 2.7.10; EC 2.7.10.1; HHF5; insulin receptor; IR

Molecular Weight

Mass (kDA):
156.333 kDA

Genomic Context

Human
Location:	19p13.2
Sequence:	19; NC_000019.10 (7112257..7294414, complement)

Tissue Specificity

Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Late endosome. Lysosome. Binding of insulin to INSR induces internalization and lysosomal degradation of the receptor, a means for downregulating this signaling pathway after stimulation. In the presence of SORL1, internalized INSR molecules are redirected back to the cell surface, thereby preventing their lysosomal catabolism and strengthening insulin signal reception.

Diseases

• Insulin Resistance
• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Malignant Neoplasms
• Obesity
• Neoplasms
• Hyperinsulinism
• Polycystic Ovary Syndrome
• Insulin Sensitivity
• Hypertensive Disease
• Hyperglycemia
• Malignant Neoplasm Of Breast
• Mammary Neoplasms

• Diabetes Mellitus, Experimental
• Essential Hypertension
• Donohue Syndrome
• Atherosclerosis
• Cell Transformation, Neoplastic
• Metabolic Syndrome X
• Metabolic Diseases

Interacting Proteins

• AGAP2
• ARRB2
• DUSP18
• ENPP1
• GRB10

• IGF1
• INS
• IRS1
• Irs1
• Irs2

• KRT31
• PIK3R1
• PLCG1
• PTPN1
• PTPN11

• PTPRR
• SH2B1
• SHC1
• Trim72

Pathways

• Secretion
• Pathogenesis
• Insulin Secretion
• Cell Proliferation
• Transport
• Glucose Homeostasis
• Aging
• Cell Growth
• Localization
• Lactation
• Dna Methylation
• Cell Cycle
• Methylation

• Cell Differentiation
• Glycolysis
• Gene Silencing
• Reverse Transcription
• Angiogenesis
• Rna Interference
• Mating

PTMs

• Phosphorylation
• Methylation
• Oxidation
• Carboxylation
• Cleavage

• Glycosylation
• Decarboxylation
• Acetylation
• Dephosphorylation
• Acylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/INSR

Boster Bio - Best Uses Of The INSR Marker

If you're wondering if Boster Bio is worth the money and why, then read on. This article will give you an overview of the INSR marker and provide the reasons why it is useful in different experiments. This article will address some of the common questions you may have regarding the INSR marker. There are also optimization tips and guides that will guide you through the process of implementing controls and optimizing your experiments. Even even if your experiments are performing well, it is possible to encounter issues. Proper controls will eliminate the majority of errors and allow you to spot and fix them.

Boster Bio Anti-IL2RA

The anti-IL2RA antibody by BosterBio is an antibody made of Rabbit Polyclonal that reacts with Human IL-2 receptor alpha (IL2RA). The price depends on how long the immunogen is however, the product's quality is outstanding. The antibody has been tested in Western Blot and Immunohistochemistry. It is available in a liquid form. The antibody is taken from human IL-2Ralpha and is available in a variety of sizes and glycerol concentrations.

The antibodies employed in IHC and WB are extremely sensitive and specific. Boster Bio has invested the last two decades in advancing technology and perfecting methods that you can rely on their products. With more than 29,000 publications, Boster Bio is a leading manufacturer of antibodies. To ensure the highest level of specificity and affinity the antibodies are evaluated against 250 different tissues and untransfected cell lines.

Boster recommends a FITC-conjugated monoclonal anti-human CD25 IL2RA monoclonal antibody. The antibody reacts with Human and is flow validated. It is available under the catalog number FC00214-FITC. Alternately, you can purchase a blocking peptide (BD Biolabs FC000261-R).