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Product Info Summary
| SKU: | A14973-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-INSM2 Antibody Picoband®
SKU/Catalog Number
A14973-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-INSM2 Antibody Picoband® catalog # A14973-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-INSM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14973-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human INSM2 recombinant protein (Position: K11-H529). Human INSM2 shares 62.4% amino acid (aa) sequence identity with mouse INSM2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14973-1 is reactive to INSM2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
52.225kDa
Background of Insm2
INSM2 may function as a growth suppressor or tumor suppressor in liver cells and in certain neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14973-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of INSM2 using anti-INSM2 antibody (A14973-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INSM2 antigen affinity purified polyclonal antibody (Catalog # A14973-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INSM2 at approximately 59 kDa. The expected band size for INSM2 is at 59 kDa.
Protein Target Info & Infographic
Gene/Protein Information For INSM2 (Source: Uniprot.org, NCBI)
Gene Name
INSM2
Full Name
Insulinoma-associated protein 2
Weight
52.225kDa
Alternative Names
insulinoma-associated 2; Nbla106; zinc finger protein IA-6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INSM2, check out the INSM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INSM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-INSM2 Antibody Picoband® (A14973-1)
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