INSIG1 Antibodies

AND INSIG1 ELISA kits, INSIG1 Proteins

Many biological assays use antibodies to detect INSIG1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with INSIG1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop INSIG1 antibodies...

We validate the specificity of these antibodies to INSIG1 by testing them on tissues known to express INSIG1 positively and negatively. Browse below to find the INSIG1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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INSIG1 Infographic by Boster Bio

All INSIG1 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Insig1 Antibody Picoband®

Mouse, Rat

Flow Cytometry, WB

$370
Cat # A02026-1
100 μg/vial

INSIG1 (NM_005542) Human Over-expression Lysate

Human

$628
Cat # LS003638
100 µg

Anti-INSIG1 Antibody Picoband®

Human

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A02026-2
100 µg/vial

INSIG1 Overview

Facts about Insulin-induced gene 1 protein.

INSIG1 3D structure. Source: alphafold

Insulin-induced gene 1 protein (INSIG1)

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs).

Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78.

Gene Information

Human
Gene Name:	INSIG1
Uniprot:	O15503
Entrez:	3638

Family

Belongs to:

INSIG family

Alternative Names

CL6; CL-6; INSIG-1 membrane protein; INSIG-1; insulin induced gene 1; insulin-induced gene 1 protein; MGC1405

Molecular Weight

Mass (kDA):
29.987 kDA

Genomic Context


Human

Location:	7q36.3
Sequence:	7; NC_000007.14 (155297772..155310235)

Tissue Specificity

Expressed in all tissues tested with highest expression in the liver.

Subcellular Localizations

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Diseases

Obesity
Diabetes Mellitus
Malignant Neoplasms
Liver Carcinoma
Diabetes Mellitus, Non-insulin-dependent
Insulin Resistance
Dyslipidemias
Neoplasms
Hypercholesterolemia
Malignant Neoplasm Of Breast
Mammary Neoplasms
Fatty Liver
Cleft Palate

Cardiovascular Diseases
Atherosclerosis
Steatohepatitis
Steatosis
Diabetes Mellitus, Experimental
Inflammation
Liver Neoplasms

Interacting Proteins

AMFR

Pathways

Cholesterol Homeostasis
Lipid Homeostasis
Lactation
Transport
Rna Interference
Proteolysis
Cell Cycle
Cell Growth
Glucose Homeostasis
Cell Proliferation
Parturition
Pathogenesis

Gene Silencing
Immune Response
Translation
Localization
Secretion
Gluconeogenesis
Mammary Gland Development

PTMs

Ubiquitination
Phosphorylation
Cleavage
Reduction

Glycosylation
Methylation
Oxidation
Geranylgeranylation

Research Areas

Cholesterol Metabolism
ER Markers
Lipid and Metabolism
Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/INSIG1

References

PMID: 9268630 by Peng Y., et al. Cloning, human chromosomal assignment, and adipose and hepatic expression of the CL-6/INSIG1 gene.

PMID: 12202038 by Yang T., et al. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER.