Product Info Summary
SKU: | A07116-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-INPP4A Antibody Picoband®
SKU/Catalog Number
A07116-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-INPP4A Antibody Picoband® catalog # A07116-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-INPP4A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07116-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
E.coli-derived human INPP4A recombinant protein (Position: Q39-E935).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07116-1 is reactive to INPP4A in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
110 kDa
Background of INPP4A
Type I inositol-3,4-bisphosphate 4-phosphatase is an enzyme that in humans is encoded by the INPP4A gene. This gene encodes an Mg++ independent enzyme that hydrolyzes the 4-position phosphate from the inositol ring of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate, and inositol 3,4-bisphosphate. Multiple transcript variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07116-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human RT4 whole cell, human MOLT-4 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: MCF-7 cell
Validation Images & Assay Conditions
![a07116 1 inpp4a primary antibodies wb testing 1 a07116 1 inpp4a primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a07116-1-inpp4a-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of INPP4A using anti-INPP4A antibody (A07116-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP4A antigen affinity purified polyclonal antibody (Catalog # A07116-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP4A at approximately 110 kDa. The expected band size for INPP4A is at 110 kDa.
![a07116 1 inpp4a primary antibodies if testing 2 a07116 1 inpp4a primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a07116-1-inpp4a-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of INPP4A using anti-INPP4A antibody (A07116-1).
INPP4A was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-INPP4A Antibody (A07116-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For INPP4A (Source: Uniprot.org, NCBI)
Gene Name
INPP4A
Full Name
Inositol polyphosphate-4-phosphatase type I A
Weight
Superfamily
inositol 3,4-bisphosphate 4-phosphatase family
Alternative Names
Inositol polyphosphate-4-phosphatase type I A INPP4A INPP4, TVAS1 inositol polyphosphate-4-phosphatase type I A inositol polyphosphate-4-phosphatase type I A|inositol polyphosphate-4-phosphatase, type I, 107kD|inositol polyphosphate-4-phosphatase, type I, 107kDa|type I inositol 3,4-bisphosphate 4-phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INPP4A, check out the INPP4A Infographic
![INPP4A infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INPP4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-INPP4A Antibody Picoband® (A07116-1)
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