Product Info Summary
SKU: | A10216 |
---|---|
Size: | 100 µg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Importin-9 IPO9 Antibody
View all Importin-9 Antibodies
SKU/Catalog Number
A10216
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-Importin-9 IPO9 Antibody (Catalog# A10216). Tested in IHC, WB application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-Importin-9 IPO9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10216)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant Importin-9 protein (amino acids 740-1,041)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A10216 is reactive to IPO9 in Human, Mouse
Reconstitution
Calculated molecular weight
115963 MW
Background of Importin-9
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10216 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 0.25-1.0 µg/ml
IHC: 5 µg/ml
Validation Images & Assay Conditions
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Immunohistochemical analysis of Importin-9. Anti-Importin-9 antibody (Respiratory Epithelium tissue at 5 &mu
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Immunohistochemical analysis of Importin-9. Anti-Importin-9 antibody in human Colon tissue at 5 &mu
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Western blot analysis of Importin-9. Anti-Importin-9 antibody was used at 0.25 &mu
Protein Target Info & Infographic
Gene/Protein Information For IPO9 (Source: Uniprot.org, NCBI)
Gene Name
IPO9
Full Name
Importin-9
Weight
115963 MW
Superfamily
importin beta family
Alternative Names
Importin-9;Imp9;Ran-binding protein 9;RanBP9;IPO9;IMP9, KIAA1192, RANBP9;HSPC273; IPO9 Imp9 importin 9 importin-9|ran-binding protein 9|ranBP9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IPO9, check out the IPO9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IPO9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Importin-9 IPO9 Antibody (A10216)
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No publications found for A10216
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Importin-9 IPO9 Antibody
Question
We are currently using anti-Importin-9 antibody A10216 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
F. Taylor
Verified customer
Asked: 2020-04-13
Answer
The anti-Importin-9 antibody (A10216) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-13
Question
Is there a BSA free version of anti-Importin-9 antibody A10216 available?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Importin-9 antibody A10216 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-26
Question
Will anti-Importin-9 antibody A10216 work for IHC with cerebellum?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
According to the expression profile of cerebellum, IPO9 is highly expressed in cerebellum. So, it is likely that anti-Importin-9 antibody A10216 will work for IHC with cerebellum.
Boster Scientific Support
Answered: 2019-07-26
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-Importin-9 antibody A10216. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
Is this A10216 anti-Importin-9 antibody reactive to the isotypes of IPO9?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The immunogen of A10216 anti-Importin-9 antibody is A partial length recombinant Importin-9 protein (amino acids 740-1,041). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-08
Question
See below the WB image, lot number and protocol we used for cerebellum using anti-Importin-9 antibody A10216. Please let me know if you require anything else.
A. Rodriguez
Verified customer
Asked: 2016-08-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-12