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Product Info Summary
| SKU: | PB9871 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IL7 Antibody Picoband®
SKU/Catalog Number
PB9871
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL7 Antibody Picoband® catalog # PB9871. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9871)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL7 recombinant protein (Position: E26-I154). Mouse IL7 shares 56.4% and 87.6% amino acid (aa) sequence identity with human and rat IL7, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9871 is reactive to Il7 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
17727 MW
Background of IL-7
Interleukin 7 (IL-7) is a protein that in humans is encoded by the IL7 gene. The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. And it is found to be a cofactor for V (D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. In addition, this cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9871 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Positive Control
WB: mouse brain tissue, mouse thymus tissue, NIH3T3 whole cell
IHC: mouse lymph nodes tissue, rat lymph nodes tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL7 using anti-IL7 antibody (PB9871).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: NIH3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9871) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20 kDa. The expected band size for IL7 is at 18 kDa.
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Figure 2. IHC analysis of IL7 using anti-IL7 antibody (PB9871).
IL7 was detected in a paraffin-embedded section of mouse lymph nodes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL7 Antibody (PB9871) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of IL7 using anti-IL7 antibody (PB9871).
IL7 was detected in a paraffin-embedded section of rat lymph nodes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL7 Antibody (PB9871) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Il7 (Source: Uniprot.org, NCBI)
Gene Name
Il7
Full Name
Interleukin-7
Weight
17727 MW
Superfamily
IL-7/IL-9 family
Alternative Names
Interleukin-7;IL-7;Il7;Il-7; IL7 IL-7 interleukin 7 interleukin-7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il7, check out the Il7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL7 Antibody Picoband® (PB9871)
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No publications found for PB9871
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Customer Q&As
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16 Customer Q&As for Anti-IL7 Antibody Picoband®
Question
Our lab want to know about using your anti-IL7 antibody for negative regulation of catalytic activity studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Thanks for your inquiry. This PB9871 anti-IL7 antibody is validated on mouse brain, nih3t3 whole cell lysates. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-29
Question
Is this PB9871 anti-IL7 antibody reactive to the isotypes of IL7?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The immunogen of PB9871 anti-IL7 antibody is E. coli-derived mouse IL7 recombinant protein (Position: E26-I154). Mouse IL7 shares 56.4% and 87.6% amino acid (aa) sequence identity with human and rat IL7, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-IL7 antibody PB9871. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
We have seen staining in mouse sperm. Are there any suggestions? Is anti-IL7 antibody supposed to stain sperm positively?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
From literature sperm does express IL7. From Uniprot.org, IL7 is expressed in sperm, pancreas, among other tissues. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-11
Question
Will anti-IL7 antibody PB9871 work on zebrafish WB with sperm?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Our lab technicians have not tested anti-IL7 antibody PB9871 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL7 antibody PB9871 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish sperm in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-10
Question
I was wanting to use your anti-IL7 antibody for WB for mouse pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse pancreas identification?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
As indicated on the product datasheet, PB9871 anti-IL7 antibody has been tested for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-03
Question
Will PB9871 anti-IL7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
It shows on the product datasheet, PB9871 anti-IL7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-24
Question
Our lab were happy with the WB result of your anti-IL7 antibody. However we have observed positive staining in sperm secreted. using this antibody. Is that expected? Could you tell me where is IL7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
From what I have seen in literature, sperm does express IL7. Generally IL7 expresses in secreted. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-02
Question
I see that the anti-IL7 antibody PB9871 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
We are currently using anti-IL7 antibody PB9871 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
The anti-IL7 antibody (PB9871) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-06
Question
See attached the WB image, lot number and protocol we used for pancreas using anti-IL7 antibody PB9871. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-23
Question
I am interested in to test anti-IL7 antibody PB9871 on mouse pancreas for research purposes, then I may be interested in using anti-IL7 antibody PB9871 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
The products we sell, including anti-IL7 antibody PB9871, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-10
Question
Does anti-IL7 antibody PB9871 work for WB with pancreas?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
According to the expression profile of pancreas, IL7 is highly expressed in pancreas. So, it is likely that anti-IL7 antibody PB9871 will work for WB with pancreas.
Boster Scientific Support
Answered: 2018-02-21
Question
Is a blocking peptide available for product anti-IL7 antibody (PB9871)?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
We do provide the blocking peptide for product anti-IL7 antibody (PB9871). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-13
Question
My question regarding product PB9871, anti-IL7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9871 anti-IL7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-14
Question
Is there a BSA free version of anti-IL7 antibody PB9871 available?
D. Wu
Verified customer
Asked: 2015-01-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL7 antibody PB9871 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-01-08
