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Product Info Summary
| SKU: | M02435 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | WB |
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Product info
Product Name
Anti- IL3 Monoclonal Antibody
SKU/Catalog Number
M02435
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IL3 Monoclonal Antibody catalog # M02435. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02435)
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIB-9
Isotype
Rat IgG1
Immunogen
COS-expressed, recombinant mouse IL-3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
M02435 is reactive to Il3 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
462529 MW
Background of IL-3
Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02435 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL3 using anti-IL3 antibody (M02435).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL3 protein 15ng,
Lane 2: recombinant mouse IL3 protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti-IL3 antigen affinity purified monoclonal antibody (Catalog # M02435) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For Il3 (Source: Uniprot.org, NCBI)
Gene Name
Il3
Full Name
Interleukin-3
Weight
462529 MW
Superfamily
IL-3 family
Alternative Names
Polycystin-1;Autosomal dominant polycystic kidney disease 1 protein;PKD1; IL3 IL-3, MCGF, MULTI-CSF interleukin 3 interleukin-3|P-cell stimulating factor|colony-stimulating factor, multiple|hematopoietic growth factor|mast-cell growth factor|multilineage-colony-stimulating factor|multipotential colony-stimulating factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il3, check out the Il3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti- IL3 Monoclonal Antibody (M02435)
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