Anti-Interleukin-3 IL3 Antibody

Product Name

Anti-Interleukin-3 IL3 Antibody

View all IL-3 Antibodies

SKU/Catalog Number

RP1046

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-3 IL3 Antibody catalog # RP1046. Tested in ELISA, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-3 IL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1046)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat IL-3 recombinant protein (Position: S28-C166). Rat IL-3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL-3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1046 is reactive to IL3 in Rat

Applications

RP1046 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

18631 MW

Background of IL-3

Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

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Anti-IL-3 antibody, RP1046, Western blotting
All lanes: Anti IL-3 (RP1046) at 0.5ug/ml
WB: Recombinant Rat IL-3 Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD

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Anti-IL-3 antibody, RP1046, Western blotting
All lanes: Anti IL-3 (RP1046) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 37KD

Gene/Protein Information For IL3 (Source: Uniprot.org, NCBI)

Alternative Names

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor IL3 IL-3, MCGF, MULTI-CSF interleukin 3 interleukin-3|P-cell stimulating factor|colony-stimulating factor, multiple|hematopoietic growth factor|mast-cell growth factor|multilineage-colony-stimulating factor|multipotential colony-stimulating factor

For more info on IL3, check out the IL3 Infographic

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In this infographic, you will see the following information for IL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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