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Product Info Summary
| SKU: | A00101-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-IL1 beta Antibody
View all IL-1 beta/IL-1F2 Antibodies
SKU/Catalog Number
A00101-3
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-IL1 beta Antibody (Catalog # A00101-3). Tested in IF, IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store Anti-IL-1 beta antibody at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL1 beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101-3)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli. The MW of recombinant mouse IL-1ß was 17 kDa.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00101-3 is reactive to IL1B in Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
46281 MW
Background of IL-1 beta/IL-1F2
IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00101-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:5,000
Flow Cytometry: User optimized
IHC: 1:50-1:250
IF Microscopy: 1:50-1:250
IP: 1:200-1:800
WB: 1:500 - 1:2,000
Neutralization: User optimized
Validation Images & Assay Conditions
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Immunofluorescence microscopy after staining of mouse carotid artery tissue with anti-Mouse IL-1β antiserum (less purified form of A00101-3) diluted 1:50.Tissue sections were prepared after cyrofixation.Reaction occurred at room temperature for 60' followed by washes and reaction with Rhodamine conjugated Gt-a-Rabbit IgG .Tissue was counterstained with bis-benzimide solution at 0.5 mg/ml in PBS for 15 min at room temperature.Panel A) shows no antibody staining of WT uninjured mouse carotid tissue.Panel B) shows anti-IL-1β staining of cells after surgical injury of tissue.Panel C) shows no antibody staining of injured carotid tissue from an IL-1β KO mouse.
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Figure 2. Western blot analysis of Il1b using anti-Il1b antibody (A00101-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Il1b antigen affinity purified polyclonal antibody (Catalog # A00101-3) at 0.5 ug/mL overnight at 4 °C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Il1b.
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Figure 3. IHC analysis of Il1b using anti-Il1b antibody (A00101-3).
Il1b was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Il1b Antibody (A00101-3) overnight at 4 °C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 °C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
46281 MW
Superfamily
IL-1 family
Alternative Names
IL-1 beta, IL-1b, Interleukin-1 beta, IL-1ß, catabolin IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1 beta Antibody (A00101-3)
Hello CJ!
A00101-3 has been cited in 15 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mast cell degranulator compound 48–80 promotes atherosclerotic plaque in apolipoprotein E knockout mice with perivascular common carotid collar placement
Effects of amygdalin on type II collagen-induced arthritis in rats
Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats
Complement 5a‐mediated trophoblasts dysfunction is involved in the development of pre‐eclampsia
Calcium-Sensing Receptor on Neutrophil Promotes Myocardial Apoptosis and Fibrosis After Acute Myocardial Infarction via NLRP3 Inflammasome Activation
Protective effects of tannic acid on acute doxorubicin-induced cardiotoxicity: Involvement of suppression in oxidative stress, inflammation, and apoptosis
Ghoneim ME,Abdallah DM,Shebl AM,El-Abhar HS. The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy. Toxicol Appl Pharmacol.2020 Oct 29
Species: Rat
A00101-3 usage in article: APP:WB, SAMPLE:PROTEIN EXTRACTIONS OF LIVER TISSUES, DILUTION:1:300
Exogenous %u03B1-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats
In vivo anti-inflammatory activity of caffeoylquinic acid derivatives from Solidago virgaurea in rats
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation
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Customer Q&As
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15 Customer Q&As for Anti-IL1 beta Antibody
Question
We have seen staining in rat lung. What should we do? Is anti-IL1 beta antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Based on literature lung does express IL1B. Based on Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2019-11-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL1 beta antibody A00101-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-23
Question
We are interested in to test anti-IL1 beta antibody A00101-3 on mouse lung for research purposes, then I may be interested in using anti-IL1 beta antibody A00101-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
The products we sell, including anti-IL1 beta antibody A00101-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-12
Question
I see that the anti-IL1 beta antibody A00101-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-08
Question
I was wanting to use your anti-IL1 beta antibody for IHC for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
You can see on the product datasheet, A00101-3 anti-IL1 beta antibody has been validated for IP, IF, IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-15
Question
We are currently using anti-IL1 beta antibody A00101-3 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
The anti-IL1 beta antibody (A00101-3) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-20
Question
I have a question about product A00101-3, anti-IL1 beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00101-3 anti-IL1 beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-05
Question
See below the WB image, lot number and protocol we used for lung using anti-IL1 beta antibody A00101-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-04
Question
Is there a BSA free version of anti-IL1 beta antibody A00101-3 available?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL1 beta antibody A00101-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-01
Question
Will A00101-3 anti-IL1 beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
It shows on the product datasheet, A00101-3 anti-IL1 beta antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-25
Question
Is a blocking peptide available for product anti-IL1 beta antibody (A00101-3)?
H. Patel
Verified customer
Asked: 2017-06-15
Answer
We do provide the blocking peptide for product anti-IL1 beta antibody (A00101-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-15
Question
Our lab want to know about using your anti-IL1 beta antibody for leukocyte migration studies. Has this antibody been tested with western blotting on carotid artery tissue? We would like to see some validation images before ordering.
C. Zhang
Verified customer
Asked: 2017-02-03
Answer
We appreciate your inquiry. This A00101-3 anti-IL1 beta antibody is validated on carotid artery tissue. It is guaranteed to work for IP, IF, IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-02-03
Question
Will anti-IL1 beta antibody A00101-3 work for IHC with lung?
P. Williams
Verified customer
Asked: 2016-06-03
Answer
According to the expression profile of lung, IL1B is highly expressed in lung. So, it is likely that anti-IL1 beta antibody A00101-3 will work for IHC with lung.
Boster Scientific Support
Answered: 2016-06-03
Question
My colleagues were satisfied with the WB result of your anti-IL1 beta antibody. However we have observed positive staining in histiocytic lymphoma cytoplasm using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
C. Zhao
Verified customer
Asked: 2013-05-30
Answer
According to literature, histiocytic lymphoma does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2013-05-30
Question
Our lab used your anti-IL1 beta antibody for IP on smooth muscle tissue in the past. I am using mouse, and I plan to use the antibody for IF next. I would like examining smooth muscle tissue as well as histiocytic lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Banerjee
Verified customer
Asked: 2013-04-23
Answer
I looked at the website and datasheets of our anti-IL1 beta antibody and it seems that A00101-3 has been validated on mouse in both IP and IF. Thus A00101-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-04-23
