Anti-Phospho-IL-7 Receptor Alpha Chain Y449 Antibody

Product Name

Anti-Phospho-IL-7 Receptor Alpha Chain Y449 Antibody

View all IL-7R alpha/CD127 Antibodies

SKU/Catalog Number

P02222

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-IL-7 Receptor Alpha Chain Y449 Antibody (Catalog # P02222). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store IL-7 Receptor Alpha Chain phospho Y449 Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-IL-7 Receptor Alpha Chain Y449 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02222)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P02222 is reactive to IL7R in Human, Mouse, Rat

Applications

P02222 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

51581 MW

Background of IL-7R alpha/CD127

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:50,000
IHC: User optimized
WB: 1:10,000

Validation Images & Assay Conditions

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Western Blot and immunoprecipitation of Rabbit anti-IL-7 Receptor alpha chain pY449 antibody. Western blot analysis of IL-7 Receptor alpha chain pY449 expression in thymocyte D1 cells (lane 1) and thymocyte D1 cells treated with IL7 (lane 2). IL-7 Receptor alpha chain pY449 at 64KD was detected using rabbit anti-IL-7 Receptor alpha chain pY449 Antigen Affinity purified polyclonal antibody (Catalog # P02222) at 1:10000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). IP: with anti-Phosphotyrosine conjugated to Protein G agarose.

Gene/Protein Information For IL7R (Source: Uniprot.org, NCBI)

Alternative Names

CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha IL7R CD127, CDW127, IL-7R-alphaA, ILRA, IL7R interleukin 7 receptor interleukin-7 receptor subunit alpha|CD127 antigen|IL-7 receptor subunit alpha|IL-7R subunit alpha|interleukin 7 receptor alpha chain

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