Anti-IL-32A Antibody Peroxidase Conjugated

Product Name

Anti-IL-32A Antibody Peroxidase Conjugated

View all IL32 Antibodies

SKU/Catalog Number

A03286-1

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-IL-32A Antibody Peroxidase Conjugated (Catalog # A03286-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-IL-32A Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03286-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Clonality

Polyclonal

Isotype

IgG

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03286-1 is reactive to IL32 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

42 kDa

Calculated molecular weight

33359 MW

Background of IL32

IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFα and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03286-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:2,500
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For IL32 (Source: Uniprot.org, NCBI)

Alternative Names

IL-32A peroxidase, interleukin-32A HRP, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32, IL-32 isoform 4 IL32 IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd interleukin 32 interleukin-32|interleukin-32 eta|interleukin-32 small|interleukin-32 theta|natural killer cell transcript 4|natural killer cells protein 4|tumor necrosis factor alpha-inducing factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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