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Product Info Summary
| SKU: | M03286 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA |
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Product info
Product Name
Anti-IL32 Antibody (monoclonal, 2I2)
SKU/Catalog Number
M03286
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL32 Antibody (monoclonal, 2I2) catalog # M03286. Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL32 Antibody (monoclonal, 2I2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03286)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2I2
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human IL32 recombinant protein (Position: A31-K234).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03286 is reactive to IL32 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
26.676kDa
Background of IL32
Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03286 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (Cap), 1-5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Sandwich ELISA - Recombinant human IL32 protein standard curve.
Use in combination with reagents from Human IL32 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1599).
Protein Target Info & Infographic
Gene/Protein Information For IL32 (Source: Uniprot.org, NCBI)
Gene Name
IL32
Full Name
Interleukin-32
Weight
26.676kDa
Alternative Names
Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF IL32 IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd interleukin 32 interleukin-32|interleukin-32 eta|interleukin-32 small|interleukin-32 theta|natural killer cell transcript 4|natural killer cells protein 4|tumor necrosis factor alpha-inducing factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL32, check out the IL32 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL32 Antibody (monoclonal, 2I2) (M03286)
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No publications found for M03286
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Customer Q&As
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1 Customer Q&As for Anti-IL32 Antibody (monoclonal, 2I2)
Question
We are currently using anti-IL32 antibody (monoclonal, 2I2) M03286 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
S. Zhao
Verified customer
Asked: 2014-07-29
Answer
The anti-IL32 antibody (monoclonal, 2I2) (M03286) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-07-29
