Anti-IL-23 Antibody

Product Name

Anti-IL-23 Antibody

View all IL-23A/IL-23 P19 Antibodies

SKU/Catalog Number

A01097

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IL-23 Antibody (Catalog # A01097). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

IL-23 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IL-23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01097)

Host

Rabbit

Contents

IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-IL-23 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of SCARB1 are known to exist; this antibody can detect both isoforms.

Reactive Species

A01097 is reactive to IL23A in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

20730 MW

Background of IL-23A/IL-23 P19

Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01097 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1-4 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL, IHC: 5 μg/mL.
Antibody validated: Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Immunohistochemistry Validation of IL-23 in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IL-23 antibody (A01097) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.