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Product Info Summary
| SKU: | A01097-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IL-23 Antibody
View all IL-23A/IL-23 P19 Antibodies
SKU/Catalog Number
A01097-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-23 Antibody (Catalog # A01097-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
IL-23 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01097-2)
Host
Rabbit
Contents
IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IL-23 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human IL-23. The immunogen is located within amino acids 40 - 90 of IL-23.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of SCARB1 are known to exist; this antibody can detect both isoforms.
Reactive Species
A01097-2 is reactive to IL23A in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20730 MW
Background of IL-23A/IL-23 P19
Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01097-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25-2 μg/mL; IF: 20 μg/mL, IHC: 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Mouse Pancreas Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: IL-23 A01097-2 (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-23 recombinant protein per lane.Antibodies: IL-23 A01097-2 (1: 0.25 μg/mL, 2: 0.5 μg/mL, 3: 1 μg/mL and 4: 2μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Tissue Lysate
Loading: 15 μg of lysates per lane. Antibodies: IL-23 A01097-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of IL-23 in Mouse Pancreas Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23 with A01097-2 at 20 μg/mL
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Immunohistochemistry Validation of IL-23 in Mouse Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IL-23 antibody (A01097-2) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Induced Expression Validation of IL-23 in Mouse Keratinocytes of Epidermis (Yoon et al.
Protein Target Info & Infographic
Gene/Protein Information For IL23A (Source: Uniprot.org, NCBI)
Gene Name
IL23A
Full Name
Interleukin-23 subunit alpha
Weight
20730 MW
Superfamily
IL-6 superfamily
Alternative Names
P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha IL23A IL-23, IL-23A, IL23P19, P19, SGRF interleukin 23 subunit alpha interleukin-23 subunit alpha|IL-23 subunit alpha|IL-23-A|IL-23p19|JKA3 induced upon T-cell activation|interleukin 23 p19 subunit|interleukin 23, alpha subunit p19|interleukin-23 subunit p19|interleukin-six, G-CSF related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL23A, check out the IL23A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL23A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-23 Antibody (A01097-2)
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