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Product Info Summary
| SKU: | A00459-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-IL-21/Il21 Antibody
SKU/Catalog Number
A00459-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-21/Il21 Antibody catalog # A00459-1. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-21/Il21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00459-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IL-21/Il21 recombinant protein (Position: H18-S146).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00459-1 is reactive to Il21 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
65-80 kDa
Calculated molecular weight
22875 MW
Background of IL-21
Interleukin 21 (IL-21) is a protein that in humans is encoded by the IL21 gene. This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00459-1 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: mouse spleen tissue, rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody (A00459-1).
IL-21/Il21 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL-21/Il21 Antibody (A00459-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody (A00459-1).
IL-21/Il21 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL-21/Il21 Antibody (A00459-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Il21 (Source: Uniprot.org, NCBI)
Gene Name
Il21
Full Name
Interleukin-21
Weight
22875 MW
Superfamily
IL-15/IL-21 family
Alternative Names
Beta-2-microglobulin; B2m IL21 CVID11, IL-21, Za11 interleukin 21 interleukin-21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il21, check out the Il21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-21/Il21 Antibody (A00459-1)
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