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Product Info Summary
| SKU: | RP1048 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Interleukin-13 IL13 Antibody
SKU/Catalog Number
RP1048
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-13 IL13 Antibody catalog # RP1048. Tested in ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-13 IL13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1048)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IL-13 recombinant protein (Position: P22-H131). Rat IL-13 shares 62% and 76% amino acid (aa) sequence identity with human and mouse IL-13, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1048 is reactive to Il13 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
14093 MW
Background of IL-13
IL-13, Interleukin 13, is a protein that in humans is encoded by the IL-13 gene. IL-13 is a kind of cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, which is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. This gene acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1048 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA(Cap), 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Sandwich ELISA - Recombinant rat IL13 protein standard curve.
Use in combination with reagents from Rat IL13 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0900).
Protein Target Info & Infographic
Gene/Protein Information For Il13 (Source: Uniprot.org, NCBI)
Gene Name
Il13
Full Name
Interleukin-13
Weight
14093 MW
Superfamily
IL-4/IL-13 family
Alternative Names
Interleukin-13;IL-13;T-cell activation protein P600;Il13;Il-13; IL13 IL-13, P600 interleukin 13 interleukin-13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il13, check out the Il13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interleukin-13 IL13 Antibody (RP1048)
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Customer Q&As
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14 Customer Q&As for Anti-Interleukin-13 IL13 Antibody
Question
I am looking for to test anti-IL13 antibody RP1048 on rat left testis for research purposes, then I may be interested in using anti-IL13 antibody RP1048 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Patel
Verified customer
Asked: 2020-04-03
Answer
The products we sell, including anti-IL13 antibody RP1048, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left testis using anti-IL13 antibody RP1048. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-25
Question
My question regarding product RP1048, anti-IL13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Huang
Verified customer
Asked: 2019-08-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1048 anti-IL13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-27
Question
Is there a BSA free version of anti-IL13 antibody RP1048 available?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL13 antibody RP1048 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-16
Question
Would anti-IL13 antibody RP1048 work for ELISA with left testis?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of left testis, IL13 is highly expressed in left testis. So, it is likely that anti-IL13 antibody RP1048 will work for ELISA with left testis.
Boster Scientific Support
Answered: 2019-07-04
Question
I see that the anti-IL13 antibody RP1048 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-21
Question
My boss were well pleased with the WB result of your anti-IL13 antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL13 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-09
Answer
From literature, blood does express IL13. Generally IL13 expresses in secreted. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2019-04-09
Question
Is this RP1048 anti-IL13 antibody reactive to the isotypes of IL13?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
The immunogen of RP1048 anti-IL13 antibody is E. coli-derived rat IL-13 recombinant protein (Position: P22-H131). Rat IL-13 shares 62% and 76% amino acid (aa) sequence identity with human and mouse IL-13, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-08
Question
I was wanting to use your anti-IL13 antibody for ELISA for rat left testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat left testis identification?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
As indicated on the product datasheet, RP1048 anti-IL13 antibody has been tested for ELISA on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat left testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-21
Question
Is a blocking peptide available for product anti-IL13 antibody (RP1048)?
Z. Roberts
Verified customer
Asked: 2018-06-15
Answer
We do provide the blocking peptide for product anti-IL13 antibody (RP1048). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-15
Question
I have attached the WB image, lot number and protocol we used for left testis using anti-IL13 antibody RP1048. Please let me know if you require anything else.
J. Anderson
Verified customer
Asked: 2018-05-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-03
Question
We have observed staining in rat blood. What should we do? Is anti-IL13 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
According to literature blood does express IL13. According to Uniprot.org, IL13 is expressed in left testis, blood, peripheral blood lymphocyte, among other tissues. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2018-04-27
Question
We are currently using anti-IL13 antibody RP1048 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on primate tissues as well?
K. Kulkarni
Verified customer
Asked: 2015-01-23
Answer
The anti-IL13 antibody (RP1048) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-23
Question
Would RP1048 anti-IL13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Parker
Verified customer
Asked: 2013-07-10
Answer
As indicated on the product datasheet, RP1048 anti-IL13 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-10
