Anti-IL1 beta/IL1B Antibody

Product Name

Anti-IL1 beta/IL1B Antibody

View all IL-1 beta/IL-1F2 Antibodies

SKU/Catalog Number

PA1533

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL1 beta/IL1B Antibody catalog # PA1533. Tested in ELISA, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL1 beta/IL1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1533)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat IL-1 beta.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1533 is reactive to IL1B in Rat

Applications

PA1533 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

31 kDa

Calculated molecular weight

30644 MW

Background of IL-1 beta/IL-1F2

Interleukin-1 beta (IL-1beta) also know as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene, which localizes to the long arm of chromosome 2 at position 2q13-2q21 between two fragile sites. Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. The human IL-1 family plays an important role in the pathogenesis of many diseases and functions as a key mediator of the host response to various infectious, inflammatory, and immunologic challenges.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.5-1μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

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Anti-IL-1 beta antibody, PA1533, Western blotting
All lanes: Anti IL-1 beta (PA1533) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Predicted bind size: 31KD
Observed bind size: 31KD

Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta

For more info on IL1B, check out the IL1B Infographic

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In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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