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Product Info Summary
| SKU: | PA1341 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL1 beta/IL1B Antibody Picoband®
View all IL-1 beta/IL-1F2 Antibodies
SKU/Catalog Number
PA1341
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL1 beta/IL1B Antibody catalog # PA1341. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1 beta/IL1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1341)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IL-1 beta.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1341 is reactive to IL1B in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
30748 MW
Background of IL-1 beta/IL-1F2
Interleukin-1 beta (IL-1beta) also know as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene, which localizes to the long arm of chromosome 2 at position 2q13-2q21 between two fragile sites. Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. The human IL-1 family plays an important role in the pathogenesis of many diseases and functions as a key mediator of the host response to various infectious, inflammatory, and immunologic challenges.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1341 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-IL-1 beta antibody, PA1341, Western blotting
Lane 1: Recombinant Human IL-1 beta Protein 20ng
Lane 2: Recombinant Human IL-1 beta Protein 10ng
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30748 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-1 beta;IL-1 beta;Catabolin;IL1B;IL1F2; IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1 beta/IL1B Antibody Picoband® (PA1341)
Hello CJ!
PA1341 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells
Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation
NLRP3 Inflammasome Sequential Changes in?Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis
Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke
Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.
Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-IL1 beta/IL1B Antibody Picoband®
Question
Does PA1341 anti-IL1 beta/IL1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
It shows on the product datasheet, PA1341 anti-IL1 beta/IL1B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-04
Question
My lab would like to test anti-IL1 beta/IL1B antibody PA1341 on human macrophage for research purposes, then I may be interested in using anti-IL1 beta/IL1B antibody PA1341 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The products we sell, including anti-IL1 beta/IL1B antibody PA1341, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-IL1 beta/IL1B antibody PA1341 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
B. Bhatt
Verified customer
Asked: 2019-12-03
Answer
The anti-IL1 beta/IL1B antibody (PA1341) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
See attached the WB image, lot number and protocol we used for macrophage using anti-IL1 beta/IL1B antibody PA1341. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-IL1 beta/IL1B antibody for WB for human macrophage on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human macrophage identification?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It shows on the product datasheet, PA1341 anti-IL1 beta/IL1B antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
Does anti-IL1 beta/IL1B antibody PA1341 work for WB with macrophage?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of macrophage, IL1B is highly expressed in macrophage. So, it is likely that anti-IL1 beta/IL1B antibody PA1341 will work for WB with macrophage.
Boster Scientific Support
Answered: 2019-09-26
Question
Is a blocking peptide available for product anti-IL1 beta/IL1B antibody (PA1341)?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We do provide the blocking peptide for product anti-IL1 beta/IL1B antibody (PA1341). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-IL1 beta/IL1B antibody PA1341. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-24
Question
My colleagues were content with the WB result of your anti-IL1 beta/IL1B antibody. However we have seen positive staining in monocyte cytosol using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
From literature, monocyte does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2019-03-12
Question
I see that the anti-IL1 beta/IL1B antibody PA1341 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-20
Question
Is this PA1341 anti-IL1 beta/IL1B antibody reactive to the isotypes of IL1B?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The immunogen of PA1341 anti-IL1 beta/IL1B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human IL-1 beta(249-269aa FLGGTKGGQDITDFTMQFVSS). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-01
Question
My question regarding product PA1341, anti-IL1 beta/IL1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1341 anti-IL1 beta/IL1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-24
Question
Do you have a BSA free version of anti-IL1 beta/IL1B antibody PA1341 available?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL1 beta/IL1B antibody PA1341 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-13
Question
We have been able to see staining in human skin. Do you have any suggestions? Is anti-IL1 beta/IL1B antibody supposed to stain skin positively?
R. Patel
Verified customer
Asked: 2016-06-22
Answer
From what I have seen in literature skin does express IL1B. From what I have seen in Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2016-06-22
