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Product Info Summary
| SKU: | A01816-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IKKi/IKKe/IKBKE Antibody Picoband®
View all IKK epsilon/IKBKE Antibodies
SKU/Catalog Number
A01816-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IKKi/IKKe/IKBKE Antibody Picoband® catalog # A01816-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKKi/IKKe/IKBKE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01816-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IKKi/IKKe/IKBKE recombinant protein (Position: Q318-V716).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01816-2 is reactive to IKBKE in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
80.462kDa
Background of IKK epsilon/IKBKE
Inhibitor of nuclear factor kappa-B kinase subunit epsilon also known as I-kappa-B kinase epsilon or IKK-epsilon is an enzyme that in humans is encoded by the IKBKE gene. IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell line.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01816-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HL-60 whole cell, human U-87MG whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKKi/IKKe/IKBKE using anti-IKKi/IKKe/IKBKE antibody (A01816-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKKi/IKKe/IKBKE antigen affinity purified polyclonal antibody (Catalog # A01816-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKKi/IKKe/IKBKE at approximately 80KD. The expected band size for IKKi/IKKe/IKBKE is at 80KD.
Protein Target Info & Infographic
Gene/Protein Information For IKBKE (Source: Uniprot.org, NCBI)
Gene Name
IKBKE
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Weight
80.462kDa
Superfamily
protein kinase superfamily
Alternative Names
Potassium voltage-gated channel subfamily A member 1; Voltage-gated K (+) channel HuKI IKBKE IKK-E, IKK-i, IKKE, IKKI inhibitor of nuclear factor kappa B kinase subunit epsilon inhibitor of nuclear factor kappa-B kinase subunit epsilon|I-kappa-B kinase epsilon|IKK-epsilon|IKK-related kinase epsilon|inducible I kappa-B kinase|inducible IkappaB kinase|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKE, check out the IKBKE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKKi/IKKe/IKBKE Antibody Picoband® (A01816-2)
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