Product Info Summary
SKU: | M01816 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IKBKE Monoclonal Antibody
View all IKK epsilon/IKBKE Antibodies
SKU/Catalog Number
M01816
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IKBKE Monoclonal Antibody catalog # M01816. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKBKE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01816)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFAO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IKKi/IKKe Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01816 is reactive to IKBKE in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
56466 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01816 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IKKi/IKKe expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IKBKE (Source: Uniprot.org, NCBI)
Gene Name
IKBKE
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Weight
56466 MW
Superfamily
protein kinase superfamily
Alternative Names
Potassium voltage-gated channel subfamily A member 1;Voltage-gated K (+) channel HuKI ;Voltage-gated potassium channel HBK1 ;Voltage-gated potassium channel subunit Kv1.1;KCNA1; IKBKE IKK-E, IKK-i, IKKE, IKKI inhibitor of nuclear factor kappa B kinase subunit epsilon inhibitor of nuclear factor kappa-B kinase subunit epsilon|I-kappa-B kinase epsilon|IKK-epsilon|IKK-related kinase epsilon|inducible I kappa-B kinase|inducible IkappaB kinase|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKE, check out the IKBKE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKBKE Monoclonal Antibody (M01816)
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Customer Q&As
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2 Customer Q&As for Anti-IKBKE Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01816?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of IKKi/IKKe expression in Raji cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-IKBKE Monoclonal antibody M01816 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-IKBKE Monoclonal antibody (M01816) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09