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Product Info Summary
| SKU: | A00148 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC |
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Product info
Product Name
Anti-IGF1 Antibody
View all IGF-I/IGF-1 Antibodies
SKU/Catalog Number
A00148
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGF1 Antibody catalog # A00148. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00148)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IGF1 recombinant protein (Position: P50-A118).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00148 is reactive to IGF1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
16550 MW
Background of IGF-I/IGF-1
Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00148 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA (Cap), 1-5μg/ml, Human
Positive Control
IHC: human pancreatic cancer tissue
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. IHC analysis of IGF1 using anti-IGF1 antibody (A00148).
IGF1 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGF1 Antibody (A00148) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-IGF1 antibody (A00148).
Overlay histogram showing Hela cells stained with A00148 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF1 Antibody (A00148, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For IGF1 (Source: Uniprot.org, NCBI)
Gene Name
IGF1
Full Name
Insulin-like growth factor I
Weight
16550 MW
Superfamily
insulin family
Alternative Names
Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5; IGF1 IGF, IGF-I, IGFI, MGF insulin like growth factor 1 insulin-like growth factor I|insulin-like growth factor 1 (somatomedin C)|insulin-like growth factor IB|mechano growth factor|somatomedin-C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGF1, check out the IGF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGF1 Antibody (A00148)
Hello CJ!
A00148 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Changes in serum IGF‑1 level and tumor VEGF expression in mice with colorectal cancer under hyperglycemic conditions
Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway
IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength
Improvement in erectile dysfunction after insulin‐like growth factor‐1 gene therapy in diabetic rats
The expressions of IGF-1, BMP-2 and TGF-β1 in cartilage of condylar hyperplasia
Polysaccharides derived from Lycium barbarum suppress IGF-1-induced angiogenesis via PI3K/HIF-1α/VEGF signalling pathways in MCF-7 cells
Development of cetuximab-resistant human nasopharyngeal carcinoma cell lines and mechanisms of drug resistance
Expression of recombinant triangular bream (Megalobrama terminalis) pre-IGF-I in Escherichia coli
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