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Product Info Summary
| SKU: | EK0378 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IGF-1 ELISA Kit PicoKine®
View all IGF-I/IGF-1 ELISA kits
SKU/Catalog Number
EK0378
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IGF-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IGF-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0378)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G49-A118
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0378 for more details
Cross-reactivity
There is no detectable cross-reactivity with IGF-2.
Reactive Species
EK0378 is reactive to Igf1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0378 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-I/IGF-1
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0378-CAP | Anti-Mouse Igf1 Pre-coated 96-well strip microplate | 1 |
| EK0378-ST | Mouse Igf1 Standard | 2 vials, 10 ng/tube |
| EK0378-DA | Mouse Igf1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.023 | 0.080 | 0.141 | 0.258 | 0.609 | 1.233 | 1.634 | 2.085 |
Data Example Images
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Mouse IGF-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-I/IGF-1, Dilution ratio of 1:100, concentration in serum and plasma is around 44-77 ng/ml..
Some articles we found to cite concentrations of IGF-I/IGF-1 in samples: 25056729 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IGF-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 75 | 604 | 1350 | 69 | 617 | 1307 |
| Standard deviation | 5.85 | 24.76 | 93.15 | 6.69 | 30.23 | 92.79 |
| CV (%) | 7.8% | 4.1% | 6.9% | 9.7% | 4.9% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGF-I/IGF-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 75 | 79 | 75 | 90 | 79 | 6.13 | 7.7% |
| Sample 2 | 604 | 579 | 666 | 572 | 605 | 37.03 | 6.1% |
| Sample 3 | 1350 | 1408 | 1325 | 1461 | 1386 | 52.73 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For Igf1 (Source: Uniprot.Org, NCBI)
Gene Name
Igf1
Full Name
Insulin-like growth factor I
Weight
15992 MW
Superfamily
insulin family
Alternative Names
Insulin-like growth factor I ;Igf1 ; IGF1 IGF, IGF-I, IGFI, MGF insulin like growth factor 1 insulin-like growth factor I|insulin-like growth factor 1 (somatomedin C)|insulin-like growth factor IB|mechano growth factor|somatomedin-C
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Igf1, check out the Igf1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Igf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IGF-1 ELISA Kit PicoKine® (EK0378)
Hello CJ!
EK0378 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Adenoviral vector expressing IGF-1 protects murine chondrogenic ATDC5 cells against hydrogen peroxide-induced mitochondrial dysfunction and apoptosis
miR-29b-3p regulated osteoblast differentiation via regulating IGF-1 secretion of mechanically stimulated osteocytes
Prkar1a haploinsufficiency ameliorates the growth hormone excess phenotype in Aip-deficient mice
Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation
Y Aghdam S,Kenchegowda D,Holmes-Hampton GP,Moroni M,P Ghosh S. Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality. Int J Mol Sci. 2021 Jan 5;22(1):451.doi:10.3390/ijms22010451.PMID:33466349;PMCID
Species: Mouse
EK0378 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:1:100
Biochemical/metabolic changes associated with hepatocellular carcinoma development in mice
Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl(2) in Qinghai-Tibetan plateau mammals and sea level mice
Adenoviral vector expressing IGF-1 protects murine chondrogenic ATDC5 cells against hydrogen peroxide-induced mitochondrial dysfunction and apoptosis
miR-29b-3p regulated osteoblast differentiation via regulating IGF-1 secretion of mechanically stimulated osteocytes
Changes in serum IGF-1 level and tumor VEGF expression in mice with colorectal cancer under hyperglycemic conditions
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Mouse IGF-1 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for IGF-I ELISA kit? I performed your IGF-I ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IGF-I even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-09-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGF-I ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IGF-I that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2020-09-29
Question
What would be a dilution rate for mice serum using EK0378?
Verified customer
Asked: 2020-09-10
Answer
The recommend dilution ratio range for mouse serum using the Mouse IGF-1 ELISA Kit PicoKine™ (EK0378) is 1:50-1:200. 60-70 ul of serum is enough for the assay after dilution.
Boster Scientific Support
Answered: 2020-09-10
Question
Does EK0378 detect free and bound IgF1?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
EK0378 detects both free and bound IGF-1.
Boster Scientific Support
Answered: 2020-04-16
Question
I want to purchase the EK0378 Mouse IGF-1 ELISA Kit. I read the catalog and it stated that the assay is used for free IGF1 measurement. I want to ask whether IGFBPs interference have been tested for this assay? You have previous validation data about this issue? Can you share it with me? Other companies suggests that IGFBPs interfere IGF1 measurement significantly.
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Yes, indeed our EK0378 Mouse IGF-1 ELISA Kit measures only free IGF-1, because IGFBPs bind up IGF-1 and restrict it from binding the assay. That
Boster Scientific Support
Answered: 2019-09-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-07-31
Question
Q: Can IGF-I ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-04-26
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IGF-I using the EK0378 Mouse IGF-I Picokine® ELISA Kit?
M. Clark
Verified customer
Asked: 2019-03-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-03-17
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IGF-I level in plasma.
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-12-14
Question
As it is illustrated in the kit protocol, the desired concentration of the target protein should fall within the standard curve (63.5pg/ml -4000pg/ml). However, my biggest concern is that I am not sure about the concentration of the target protein in my serum sample, therefore I don
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
We can not provide extra well strips because it is useless to provide extra strips without providing extra other components in the whole package. Running pilot test to figure out the optimal dilution ratio is a part of the whole experiment. Our suggested dilution ratio for serum from healthy mouse is 1:10-1:100.
Boster Scientific Support
Answered: 2017-08-31
Question
In your instructions you state that 98% of the IGF-1 produced is bound by IGF binding proteins but the standard in your kit is purified recombinant material. Do the antibodies in your kit binding IGF-1 when it is bound to binding proteins or is your kit just measuring the 2% of the IGF that isn't bound to binding proteins? If one wanted to measure total IGF-1, how would you recommend one prepare the serum and plasma samples, let alone the cell supernatants? Does this ELISA work with RIPA treated samples for instance and if so do you have any data? Keyword: applications, protocol, detection
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
Our mouse IGF-1 Elisa kit can detect both free and bound IGF-1 protein, it is no use to pretreat serum and plasma. It is good to know that one does not have to pretreat the samples or remove the IGF-1 binding proteins for your assay.
Boster Scientific Support
Answered: 2017-07-19
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
L. Kapoor
Verified customer
Asked: 2016-05-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-05-01
Question
Q: how can I thaw whole blood sample for IGF-I ELISA after freezing?
C. Thompson
Verified customer
Asked: 2014-09-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-I for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2014-09-02
