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Product Info Summary
| SKU: | A05930-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-IGBP1 Antibody Picoband®
SKU/Catalog Number
A05930-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGBP1 Antibody Picoband® catalog # A05930-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IGBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05930-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IGBP1 recombinant protein (Position: D47-I208).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05930-2 is reactive to IGBP1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
39.222kDa
Background of IGBP1
Immunoglobulin-binding protein 1 is a protein that in humans is encoded by the IGBP1 gene. The proliferation and differentiation of B cells is dependent upon a B-cell antigen receptor (BCR) complex. Binding of antigens to specific B-cell receptors results in a tyrosine phosphorylation reaction through the BCR complex and leads to multiple signal transduction pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05930-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human A549 whole cell, human Hela whole cell
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IGBP1 using anti-IGBP1 antibody (A05930-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGBP1 antigen affinity purified polyclonal antibody (Catalog # A05930-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGBP1 at approximately 45 kDa. The expected band size for IGBP1 is at 45 kDa.
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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-IGBP1 antibody (A05930-2).
Overlay histogram showing PC-3 cells stained with A05930-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IGBP1 Antibody (A05930-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For IGBP1 (Source: Uniprot.org, NCBI)
Gene Name
IGBP1
Full Name
Immunoglobulin-binding protein 1
Weight
39.222kDa
Superfamily
IGBP1/TAP42 family
Alternative Names
B-cell CLL/lymphoma 9-like protein; B-cell lymphoma 9-like protein; BCL9-like protein; Protein BCL9-2; BCL9L; DLNB11 IGBP1 ALPHA-4, IBP1, alpha4 immunoglobulin binding protein 1 immunoglobulin-binding protein 1|B cell signal transduction molecule alpha 4|CD79a-binding protein 1|bA351K23.1 (immunoglobulin binding protein 1 (CD79A) )|immunoglobulin (CD79A) binding protein 1|protein alpha-4|protein phosphatase 2/4/6 regulatory subunit|protein phosphatase 2A, regulatory subunit alpha-4|renal carcinoma NY-REN-16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGBP1, check out the IGBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGBP1 Antibody Picoband® (A05930-2)
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