Anti-IDO1/Ido Monoclonal Antibody

Product Name

Anti-IDO1/Ido Monoclonal Antibody

View all Indoleamine 2,3-dioxygenase/IDO Antibodies

SKU/Catalog Number

M01705

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-IDO1/Ido Monoclonal Antibody (Catalog # M01705). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-IDO1/Ido Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01705)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 2E2.6 IgG1

Isotype

IgG1

Immunogen

IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01705 is reactive to IDO1 in Mouse

Reconstitution

Calculated molecular weight

45326 MW

Background of Indoleamine 2,3-dioxygenase/IDO

Anti-IDO-1 antibody recognizes indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M01705 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000-1:50000
Flow Cytometry: 0.5-1x10^6 cells
IHC: User optimized
IF Microscopy: 1:50-1:100
IP: 10-100 µL
WB: 1:500-1:1500

Validation Images & Assay Conditions

Gene/Protein Information For IDO1 (Source: Uniprot.org, NCBI)

Alternative Names

IDO1, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido 1, Ido-1, IDO1, IDO-1 IDO1 IDO, IDO-1, INDO indoleamine 2,3-dioxygenase 1 indoleamine 2,3-dioxygenase 1|indolamine 2,3 dioxygenase|indole 2,3-dioxygenase|indoleamine-pyrrole 2,3-dioxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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