Anti-ICMT Antibody Picoband™

Product Name

Anti-ICMT Antibody Picoband™

View all ICMT Antibodies

SKU/Catalog Number

A06174-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-ICMT Antibody Picoband™ catalog # A06174-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ICMT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06174-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human ICMT recombinant protein (Position: E13-L284). Human ICMT shares 96.3% and 95.2% amino acid (aa) sequence identity with mouse and rat ICMT, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06174-1 is reactive to ICMT in Human, Rat

Applications

A06174-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

52588 MW

Background of ICMT

Protein-S-isoprenylcysteine O-methyltransferase is an enzyme that in humans is encoded by the ICMT gene. This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ICMT using anti-ICMT antibody (A06174-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICMT antigen affinity purified polyclonal antibody (Catalog # A06174-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICMT at approximately 32 kDa. The expected band size for ICMT is at 32 kDa.

Gene/Protein Information For ICMT (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1.100; HSTE14; Isoprenylcysteine Carboxyl Methyltransferase; Isoprenylcysteine carboxylmethyltransferase; MST098; MSTP098; PCCMT; PCMT; PPMT; Prenylated Protein Carboxyl Methyltransferase; Protein-S-Isoprenylcysteine O-Methyltransferase ICMT HSTE14, MST098, MSTP098, PCCMT, PCMT, PPMT isoprenylcysteine carboxyl methyltransferase protein-S-isoprenylcysteine O-methyltransferase|prenylated protein carboxyl methyltransferase|prenylcysteine carboxyl methyltransferase

For more info on ICMT, check out the ICMT Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ICMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ICMT Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis