Product Info Summary
SKU: | A13393 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Checkpoint protein HUS1B Hus1B Antibody
SKU/Catalog Number
A13393
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Checkpoint protein HUS1B Hus1B Antibody (Catalog # A13393). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Checkpoint protein HUS1B Hus1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13393)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human Hus1B protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13393 is reactive to HUS1B in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
30982 MW
Background of HUS1B
The protein encoded by the Hus1B gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the checkpoint protein RAD1 but not with RAD9. Over expression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to HUS1. Hus1B is expressed strongly in testis, less in spleen, thymus, prostate, colon and leukocytes. Anti-HUSB1 Antibody is useful for researchers interested in DNA damage and repair.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13393 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:100,000
IP: 1:100
WB: 1:500 - 1:5,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Hus1B expression in HELA cell lysate (arrowhead). Hus1B at 36KD was detected using rabbit anti-Hus1B Antigen Affinity purified polyclonal antibody (Catalog # A13393). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Personal Communication, A-Lien Lu-Chang, U. Maryland, Baltimore, MD
Protein Target Info & Infographic
Gene/Protein Information For HUS1B (Source: Uniprot.org, NCBI)
Gene Name
HUS1B
Full Name
Checkpoint protein HUS1B
Weight
30982 MW
Superfamily
HUS1 family
Alternative Names
HUS1B, Checkpoint protein HUS1B, hHUS1B, HUS 1B, HUS-1B, HUS1 Checkpoint Clamp Component B, HUS1B, HUS1 (S. Pombe) Checkpoint Homolog B, HUS1 Checkpoint Homolog B (S. Pombe), HUS1 Checkpoint Homolog B HUS1B HUS1 checkpoint clamp component B checkpoint protein HUS1B|HUS1 checkpoint homolog b|hHUS1B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HUS1B, check out the HUS1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HUS1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Checkpoint protein HUS1B Hus1B Antibody (A13393)
Hello CJ!
A13393 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Investigation of the Possible Role of RAD9 in Post-Diapaused Embryonic Development of the Brine Shrimp Artemia sinica
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Customer Q&As
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6 Customer Q&As for Anti-Checkpoint protein HUS1B Hus1B Antibody
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Hus1B antibody A13393. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
Does anti-Hus1B antibody A13393 work for IP with liver?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of liver, HUS1B is highly expressed in liver. So, it is likely that anti-Hus1B antibody A13393 will work for IP with liver.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-Hus1B antibody A13393 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
The anti-Hus1B antibody (A13393) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-01
Question
Would anti-Hus1B antibody A13393 work on primate IP with liver?
H. Banerjee
Verified customer
Asked: 2017-10-16
Answer
Our lab technicians have not tested anti-Hus1B antibody A13393 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Hus1B antibody A13393 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-16
Question
I was wanting to use your anti-Hus1B antibody for IP for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
F. Banerjee
Verified customer
Asked: 2016-05-20
Answer
As indicated on the product datasheet, A13393 anti-Hus1B antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-20
Question
Is this A13393 anti-Hus1B antibody reactive to the isotypes of HUS1B?
K. Moore
Verified customer
Asked: 2014-06-03
Answer
The immunogen of A13393 anti-Hus1B antibody is A synthetic peptide corresponding to amino acids 104-117 of human Hus1B protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-06-03