Product Info Summary
SKU: | A10111 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-ALX3 Antibody
SKU/Catalog Number
A10111
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ALX3 Antibody (Catalog # A10111). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALX3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10111)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ALX3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10111 is reactive to ALX3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
36935 MW
Background of ALX3
Transcriptional regulator with a possible role in patterning of mesoderm during development By similarity.
Wimmer K., Genes Chromosomes Cancer 33:285-294(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10111 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 293 cells, using ALX3 antibody A10111.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using ALX3 antibody A10111.
Protein Target Info & Infographic
Gene/Protein Information For ALX3 (Source: Uniprot.org, NCBI)
Gene Name
ALX3
Full Name
Homeobox protein aristaless-like 3
Weight
36935 MW
Superfamily
paired homeobox family
Alternative Names
Homeobox protein aristaless-like 3;Proline-rich transcription factor ALX3;ALX3; ALX3 FND, FND1 ALX homeobox 3 homeobox protein aristaless-like 3|aristaless-like homeobox 3|frontonasal dysplasia|proline-rich transcription factor ALX3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALX3, check out the ALX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALX3 Antibody (A10111)
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No publications found for A10111
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Customer Q&As
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6 Customer Q&As for Anti-ALX3 Antibody
Question
I see that the anti-ALX3 antibody A10111 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-26
Question
Is this A10111 anti-ALX3 antibody reactive to the isotypes of ALX3?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The immunogen of A10111 anti-ALX3 antibody is Synthesized peptide derived from internal of human ALX3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-25
Question
Is a blocking peptide available for product anti-ALX3 antibody (A10111)?
J. Gonzalez
Verified customer
Asked: 2018-01-01
Answer
We do provide the blocking peptide for product anti-ALX3 antibody (A10111). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-01
Question
Does A10111 anti-ALX3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
It shows on the product datasheet, A10111 anti-ALX3 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-11
Question
We are currently using anti-ALX3 antibody A10111 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
N. Gonzalez
Verified customer
Asked: 2015-01-19
Answer
The anti-ALX3 antibody (A10111) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-19
Question
Is there a BSA free version of anti-ALX3 antibody A10111 available?
D. Zhang
Verified customer
Asked: 2013-05-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ALX3 antibody A10111 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-20