Anti-human peroxidasin homolog isoform X1 Antibody

Figure 1. Western blot analysis of Peroxidasin homolog isoform X1 using anti-Peroxidasin homolog isoform X1 antibody (DZ33984).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates
Lane 2: human A375 whole cell lysates
Lane 3: human T47D whole cell lysates
Lane 4: human MDA-MB-453 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxidasin homolog isoform X1 antigen affinity purified polyclonal antibody (Catalog # DZ33984) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

Figure 2. IHC analysis of Peroxidasin homolog isoform X1 using anti-Peroxidasin homolog isoform X1 antibody (DZ33984).
Peroxidasin homolog isoform X1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peroxidasin homolog isoform X1 Antibody (DZ33984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.