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Product Info Summary
| SKU: | A03918-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Perilipin A DyLight® 550 conjugated PLIN1 Antibody
SKU/Catalog Number
A03918-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Perilipin A DyLight® 550 conjugated PLIN1 Antibody catalog # A03918-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Perilipin A DyLight® 550 conjugated PLIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03918-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Perilipin A, which shares 80% amino acid (aa) sequence identity with both mouse and rat Perilipin A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03918-Dyl550 is reactive to PLIN1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
55.99kDa
Background of Perilipin
Perilipin, also known as lipid droplet-associated protein or PLIN, is a protein that, in humans, is encoded by the PLIN gene. The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03918-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For PLIN1 (Source: Uniprot.org, NCBI)
Gene Name
PLIN1
Full Name
Perilipin-1
Weight
55.99kDa
Superfamily
perilipin family
Alternative Names
Perilipin-1; Lipid droplet-associated protein; PLIN1; PERI; PLIN PLIN1 FPLD4, PERI, PLIN perilipin 1 perilipin-1|lipid droplet-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLIN1, check out the PLIN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Perilipin A DyLight® 550 conjugated PLIN1 Antibody (A03918-Dyl550)
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No publications found for A03918-Dyl550
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Perilipin A DyLight® 550 conjugated PLIN1 Antibody
Question
I was wanting to use your anti-Human Perilipin A DyLight® 550 conjugated antibody for Flow Cytometry for human adipose tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adipose tissue identification?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
It shows on the product datasheet, A03918-Dyl550 anti-Human Perilipin A DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human adipose tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-23
Question
Do you have a BSA free version of anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 available?
F. Taylor
Verified customer
Asked: 2020-04-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
We are currently using anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The anti-Human Perilipin A DyLight® 550 conjugated antibody (A03918-Dyl550) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-09
Question
Is a blocking peptide available for product anti-Human Perilipin A DyLight® 550 conjugated antibody (A03918-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We do provide the blocking peptide for product anti-Human Perilipin A DyLight® 550 conjugated antibody (A03918-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-29
Question
I see that the anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-22
Question
Will A03918-Dyl550 anti-Human Perilipin A DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can see on the product datasheet, A03918-Dyl550 anti-Human Perilipin A DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-06
Question
Can you help my question with product A03918-Dyl550, anti-Human Perilipin A DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03918-Dyl550 anti-Human Perilipin A DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-05
Question
My team were happy with the WB result of your anti-Human Perilipin A DyLight® 550 conjugated antibody. However we have observed positive staining in adipose tissue endoplasmic reticulum using this antibody. Is that expected? Could you tell me where is PLIN1 supposed to be expressed?
M. Kulkarni
Verified customer
Asked: 2019-08-13
Answer
According to literature, adipose tissue does express PLIN1. Generally PLIN1 expresses in endoplasmic reticulum. Regarding which tissues have PLIN1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 9521880
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-13
Question
Would anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 work for Flow Cytometry with adipose tissue?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
According to the expression profile of adipose tissue, PLIN1 is highly expressed in adipose tissue. So, it is likely that anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 will work for Flow Cytometry with adipose tissue.
Boster Scientific Support
Answered: 2019-07-02
Question
Is this A03918-Dyl550 anti-Human Perilipin A DyLight® 550 conjugated antibody reactive to the isotypes of PLIN1?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
The immunogen of A03918-Dyl550 anti-Human Perilipin A DyLight® 550 conjugated antibody is A synthetic peptide corresponding to a sequence of human Perilipin A (DKVLGAALAGCELAWGVARDTAEFAANTRAGRLAS). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-15
Question
See below the WB image, lot number and protocol we used for adipose tissue using anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550. Please let me know if you require anything else.
P. Lewis
Verified customer
Asked: 2016-04-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-19
Question
We have seen staining in human brain. Any tips? Is anti-Human Perilipin A DyLight® 550 conjugated antibody supposed to stain brain positively?
E. Evans
Verified customer
Asked: 2014-01-29
Answer
Based on literature brain does express PLIN1. Based on Uniprot.org, PLIN1 is expressed in adipose tissue, adipocyte, brain, liver, among other tissues. Regarding which tissues have PLIN1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 9521880
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2014-01-29
Question
We are interested in to test anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 on human adipose tissue for research purposes, then I may be interested in using anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Thomas
Verified customer
Asked: 2013-04-08
Answer
The products we sell, including anti-Human Perilipin A DyLight® 550 conjugated antibody A03918-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-08
