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Product Info Summary
| SKU: | M01394-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Human IBA1 AIF1 Antibody
View all AIF-1/Iba1 Antibodies
SKU/Catalog Number
M01394-2
Size
100ul
Description
Boster Bio Anti-Human IBA1 AIF1 Antibody catalog # M01394-2. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IBA1 AIF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394-2)
Host
Rabbit
Contents
Supplied as an aliquot of serum plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Peptide identical to part of the C-terminal of human IBA1 coupled to KLH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01394-2 is reactive to AIF1 in Human
Reconstitution
Calculated molecular weight
16703 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01394-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-5,000. IF 1:2,000-5,000
Validation Images & Assay Conditions
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Western blot analysis of different tissue lysates using rabbit pAb to IBA1, M01394-2, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain, [4] mouse spleen, and [5] rat spleen. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates. The other bands seen in the CNS lysates are of unknown origin but do not appear to compromise the migroglial specific staining seen with this antibody.
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High magnification stacked confocal image of rat cerebellar molecular layer at top and granular layer below, stained with M01394-2, dilution 1:1,000, in green. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Red shows the processes of Purkinje cells and the perikarya of granule cells revealed with , an antibody to MAP2, 1:5,000. Nuclear DNA is shown with DAPI stain in blue. This image is part of one of our high resolution poster images, see .
Protein Target Info & Infographic
Gene/Protein Information For AIF1 (Source: Uniprot.org, NCBI)
Gene Name
AIF1
Full Name
Allograft inflammatory factor 1
Weight
16703 MW
Alternative Names
Allograft inflammatory factor 1;AIF-1;Ionized calcium-binding adapter molecule 1;Protein G1;AIF1;G1, IBA1; AIF1 AIF-1, IBA1, IRT-1, IRT1 allograft inflammatory factor 1 allograft inflammatory factor 1|interferon gamma responsive transcript|ionized calcium-binding adapter molecule 1|protein G1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AIF1, check out the AIF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AIF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IBA1 AIF1 Antibody (M01394-2)
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Customer Q&As
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16 Customer Q&As for Anti-Human IBA1 AIF1 Antibody
Question
Is there a BSA free version of anti-Human IBA1 antibody M01394-2 available?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human IBA1 antibody M01394-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-06
Question
I have a question about product M01394-2, anti-Human IBA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01394-2 anti-Human IBA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-05
Question
Does M01394-2 anti-Human IBA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
It shows on the product datasheet, M01394-2 anti-Human IBA1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Human IBA1 antibody M01394-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
I was wanting to use your anti-Human IBA1 antibody for IHC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It shows on the product datasheet, M01394-2 anti-Human IBA1 antibody has been tested for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
We have observed staining in human liver. Any tips? Is anti-Human IBA1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
From literature liver does express AIF1. From Uniprot.org, AIF1 is expressed in metanephric glomerulus, lymphocyte, glial tumor, prostate, erythroleukemia, liver, among other tissues. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-27
Question
Will anti-Human IBA1 antibody M01394-2 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
According to the expression profile of liver, AIF1 is highly expressed in liver. So, it is likely that anti-Human IBA1 antibody M01394-2 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-08-22
Question
We ordered your anti-Human IBA1 antibody for IHC on prostate in a previous project. I am using human, and We are going to use the antibody for IF next. I was wanting to use examining prostate as well as erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
I looked at the website and datasheets of our anti-Human IBA1 antibody and I see that M01394-2 has been tested on human in both IHC and IF. Thus M01394-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-17
Question
Please see the WB image, lot number and protocol we used for liver using anti-Human IBA1 antibody M01394-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-26
Question
I see that the anti-Human IBA1 antibody M01394-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-01
Question
I am interested in using your anti-Human IBA1 antibody for negative regulation of apoptotic process studies. Has this antibody been tested with western blotting on granule cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
Thank you for your inquiry. This M01394-2 anti-Human IBA1 antibody is validated on mouse brain, granule cells, purkinje cells. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-11-15
Question
I am looking for to test anti-Human IBA1 antibody M01394-2 on human liver for research purposes, then I may be interested in using anti-Human IBA1 antibody M01394-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
The products we sell, including anti-Human IBA1 antibody M01394-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-30
Question
We are currently using anti-Human IBA1 antibody M01394-2 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
The anti-Human IBA1 antibody (M01394-2) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-09
Question
We were satisfied with the WB result of your anti-Human IBA1 antibody. However we have observed positive staining in lymphocyte cytoplasm using this antibody. Is that expected? Could you tell me where is AIF1 supposed to be expressed?
D. Carter
Verified customer
Asked: 2016-05-25
Answer
From literature, lymphocyte does express AIF1. Generally AIF1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-05-25
Question
Is this M01394-2 anti-Human IBA1 antibody reactive to the isotypes of AIF1?
E. Roberts
Verified customer
Asked: 2015-01-06
Answer
The immunogen of M01394-2 anti-Human IBA1 antibody is Peptide identical to part of the C-terminal of human IBA1 coupled to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-06
Question
Is a blocking peptide available for product anti-Human IBA1 antibody (M01394-2)?
B. Krishna
Verified customer
Asked: 2014-10-20
Answer
We do provide the blocking peptide for product anti-Human IBA1 antibody (M01394-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-10-20
