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Product Info Summary
| SKU: | M01180-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody(monoclonal, 5G3)
SKU/Catalog Number
M01180-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody (monoclonal, 5G3) catalog # M01180-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody(monoclonal, 5G3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01180-Dyl550)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
5G3
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01180-Dyl550 is reactive to XPO1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
123.386kDa
Background of CRM1
Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01180-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For XPO1 (Source: Uniprot.org, NCBI)
Gene Name
XPO1
Full Name
Exportin-1
Weight
123.386kDa
Superfamily
exportin family
Alternative Names
Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog; XPO1; CRM1 XPO1 CRM-1, CRM1, emb, exp1 exportin 1 exportin-1|chromosome region maintenance 1 homolog|chromosome region maintenance 1 protein homolog|exportin 1 (CRM1 homolog, yeast)|exportin-1 (required for chromosome region maintenance)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XPO1, check out the XPO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XPO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody(monoclonal, 5G3) (M01180-Dyl550)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody(monoclonal, 5G3)
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
I see that the anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-17
Question
Do you have a BSA free version of anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 available?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-17
Question
Does anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 work on canine Flow Cytometry with liver?
R. Anderson
Verified customer
Asked: 2019-03-20
Answer
Our lab technicians have not tested anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-20
Question
Will anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 work for Flow Cytometry with placenta?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
According to the expression profile of placenta, XPO1 is highly expressed in placenta. So, it is likely that anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) M01180-Dyl550 will work for Flow Cytometry with placenta.
Boster Scientific Support
Answered: 2018-03-20
Question
I have a question about product M01180-Dyl550, anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01180-Dyl550 anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-25
Question
Is this M01180-Dyl550 anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) reactive to the isotypes of XPO1?
V. Jackson
Verified customer
Asked: 2015-10-12
Answer
The immunogen of M01180-Dyl550 anti-Human CRM1 DyLight® 550 conjugated antibody(monoclonal, 5G3) is E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-10-12
