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Product Info Summary
| SKU: | M04544-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6)
SKU/Catalog Number
M04544-Dyl488
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Human COPE DyLight® 488 conjugated Antibody (monoclonal, 9B6) catalog # M04544-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04544-Dyl488)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
9B6
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M04544-Dyl488 is reactive to COPE in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
34.482kDa
Background of COPE
Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04544-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of A431 cells using anti-Human COPE antibody (M04544-Dyl488).
Overlay histogram showing A431 cells stained with M04544-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human COPE Antibody (M04544-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For COPE (Source: Uniprot.org, NCBI)
Gene Name
COPE
Full Name
Coatomer subunit epsilon
Weight
34.482kDa
Superfamily
COPE family
Alternative Names
Coatomer subunit epsilon; Epsilon-coat protein; Epsilon-COP; COPE COPE epsilon-COP COPI coat complex subunit epsilon coatomer subunit epsilon|coatomer epsilon subunit|coatomer protein complex subunit epsilon|epsilon coat protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COPE , check out the COPE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COPE : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6) (M04544-Dyl488)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6)
Question
We are currently using anti-Human COPE DyLight® 488 conjugated antibody(monoclonal, 9B6) M04544-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
D. Dhar
Verified customer
Asked: 2016-10-11
Answer
The anti-Human COPE DyLight® 488 conjugated antibody(monoclonal, 9B6) (M04544-Dyl488) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-11
