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Product Info Summary
| SKU: | FC01281 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD81 Monoclonal Antibody, Flow Validated
SKU/Catalog Number
FC01281
Size
100ug, 500ug
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD81 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC01281). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD81 Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01281)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 1D6
Isotype
IgG1,k
Immunogen
5A6 aggregated OCI-Ly8 human B cell line
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01281 is reactive to CD81 in Human
Reconstitution
Calculated molecular weight
25809 MW
Background of CD81
Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01281 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained with purified anti-human CD81 (clone 1D6) followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocytes gated PBMCs stained with mouse IgG1 isotype control followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram). The data is generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For CD81 (Source: Uniprot.org, NCBI)
Gene Name
CD81
Full Name
CD81 antigen
Weight
25809 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD81 antigen;26 kDa cell surface protein TAPA-1;Target of the antiproliferative antibody 1;Tetraspanin-28;Tspan-28;CD81;CD81;TAPA1, TSPAN28; CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 |26 kDa cell surface protein TAPA-1|CD81 (target of antiproliferative 1)|tetraspanin-28|tspan-28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD81, check out the CD81 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD81 Monoclonal Antibody, Flow Validated (FC01281)
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No publications found for FC01281
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-Human CD81 Monoclonal Antibody, Flow Validated
Question
Will anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 work for Flow Cytometry with myometrium?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
According to the expression profile of myometrium, CD81 is highly expressed in myometrium. So, it is likely that anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 will work for Flow Cytometry with myometrium.
Boster Scientific Support
Answered: 2019-05-27
Question
Do you have a BSA free version of anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 available?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-24
Question
Is a blocking peptide available for product anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated (FC01281)?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
We do provide the blocking peptide for product anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated (FC01281). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-18
Question
I have a question about product FC01281, anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Carter
Verified customer
Asked: 2018-09-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01281 anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-11
Question
Would FC01281 anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
As indicated on the product datasheet, FC01281 anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-06
Question
I am looking for to test anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 on human myometrium for research purposes, then I may be interested in using anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The products we sell, including anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-06
Question
I was wanting to use your anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated for Flow Cytometry for human myometrium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human myometrium identification?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
You can see on the product datasheet, FC01281 anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human myometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-16
Question
Is this FC01281 anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated reactive to the isotypes of CD81?
S. Krishna
Verified customer
Asked: 2016-11-11
Answer
The immunogen of FC01281 anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated is 5A6 aggregated OCI-Ly8 human B cell line. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-11-11
Question
I see that the anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
N. Parker
Verified customer
Asked: 2015-07-07
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-07-07
Question
I have attached the WB image, lot number and protocol we used for myometrium using anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281. Please let me know if you require anything else.
M. Parker
Verified customer
Asked: 2013-08-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myometrium using anti-Human CD81 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC01281. Let me know if you need anything else.
B. Krishna
Verified customer
Asked: 2013-06-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-27
