Click image to see more details
Product Info Summary
| SKU: | FC01974-1-Biotin |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated
SKU/Catalog Number
FC01974-1-Biotin
Size
100ug
Form
Liquid
Description
Boster Bio Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC01974-1-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01974-1-Biotin)
Host
Mouse
Contents
PBS pH 7.2, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
Clone: 124-1D1
Isotype
IgG1,k
Immunogen
Human Leukocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01974-1-Biotin is reactive to CD7 in Human
Reconstitution
Calculated molecular weight
25409 MW
Background of CD7
CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01974-1-Biotin is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated PBMCs stained with biotin conjugated anti-human CD7 (clone 4H9) followed by staining with SA-PE (red histogram). Lymphocytes gated PBMCs stained with biotin conjugated mouse IgG2a isotype control followed by staining with SAPE (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD7 (Source: Uniprot.org, NCBI)
Gene Name
CD7
Full Name
T-cell antigen CD7
Weight
25409 MW
Alternative Names
T-cell antigen CD7;GP40;T-cell leukemia antigen;T-cell surface antigen Leu-9;TP41;CD7;CD7; CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell CD7|CD7 (p41)|T-cell leukemia |T-cell surface Leu-9|p41 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD7, check out the CD7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated (FC01974-1-Biotin)
Hello CJ!
FC01974-1-Biotin has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Human papillomavirus genotypes and p16 expression in oral leukoplakia and squamous cell carcinoma Li-Qun Yang,1,2,3,4,* Xuan Xiao,5,* Chen-Xi Li,1,2 Wen-Yan Wu,1,2 Xue-Min Shen,1,2 Zeng-Tong Zhou,1,2 Yuan Fan,3,4 Lin-Jun Shi1,2 Int J Clin Exp Pathol.
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated
Question
Will FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
You can see on the product datasheet, FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-26
Question
See attached the WB image, lot number and protocol we used for muscle using Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
Do you have a BSA free version of Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin available?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
I see that the Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
P. Anderson
Verified customer
Asked: 2018-10-11
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-11
Question
Is this FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated reactive to the isotypes of CD7?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The immunogen of FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated is Human Leukocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-02
Question
Will Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin work for Flow Cytometry with muscle?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
According to the expression profile of muscle, CD7 is highly expressed in muscle. So, it is likely that Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin will work for Flow Cytometry with muscle.
Boster Scientific Support
Answered: 2018-05-24
Question
I would like to test Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin on human muscle for research purposes, then I may be interested in using Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
The products we sell, including Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-26
Question
I was wanting to use your Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated for Flow Cytometry for human muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human muscle identification?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
It shows on the product datasheet, FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-11
Question
I have a question about product FC01974-1-Biotin, Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Verified customer
Asked: 2014-04-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin. Let me know if you need anything else.
E. Wu
Verified customer
Asked: 2014-03-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-13
Question
Is a blocking peptide available for product Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01974-1-Biotin)?
T. Krishna
Verified customer
Asked: 2013-07-31
Answer
We do provide the blocking peptide for product Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01974-1-Biotin). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-31
