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Product Info Summary
| SKU: | FC00171 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD54 Monoclonal Antibody, Flow Validated
View all ICAM-1/CD54 Antibodies
SKU/Catalog Number
FC00171
Size
100ug, 500ug
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD54 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC00171). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD54 Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00171)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: LB-2
Isotype
IgG2b,k
Immunogen
MHC Class II antigen negative human Blymphoblastoid cell line
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00171 is reactive to Icam1 in Human
Reconstitution
Calculated molecular weight
60142 MW
Background of ICAM-1/CD54
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00171 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Daudi gated cells stained with purified mouse anti-human CD54 (clone LB-2) followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 staining (red histogram). Daudi gated cells stained with purified mouse IgG2b isotype control followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 staining (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For Icam1 (Source: Uniprot.org, NCBI)
Gene Name
Icam1
Full Name
Intercellular adhesion molecule 1
Weight
60142 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Intercellular adhesion molecule 1;ICAM-1;CD54;Icam1;Icam-1; ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Icam1, check out the Icam1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Icam1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD54 Monoclonal Antibody, Flow Validated (FC00171)
Hello CJ!
FC00171 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Cheng Y, Zhang Y, Song H, Feng J. Neural Regen Res. 2012 Jun 15;7(17):1312-7. Doi: 10.3969/J.Issn.1673-5374.2012.17.005. Intercellular Adhesion Molecule-1 Expression In The Hippocampal Ca1 Region Of Hyperlipidemic Rats With Chronic Cerebral Ischemia.
Yao Jh, Zhang Xs, Zheng Ss, Li Yh, Wang Lm, Wang Zz, Chu L, Hu Xw, Liu Kx, Tian Xf. World J Gastroenterol. 2009 Jul 14;15(26):3240-5. Prophylaxis With Carnosol Attenuates Liver Injury Induced By Intestinal Ischemia/Reperfusion.
Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H. Crit Care. 2013 Mar 11;17(2):R44. Doi: 10.1186/Cc12558. Glycyrrhizin Protects Against Porcine Endotoxemia Through Modulation Of Systemic Inflammatory Response.
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