Click image to see more details
Product Info Summary
| SKU: | FC00555-mFluor540 |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated
SKU/Catalog Number
FC00555-mFluor540
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated (Catalog# FC00555-mFluor540). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00555-mFluor540)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: F10-89-4
Isotype
IgG2a,k
Immunogen
Human T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00555-mFluor540 is reactive to PTPRC in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
147254 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00555-mFluor540 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated blood from healthy volunteer stained with mFluor540 conjugated anti-human CD45 (clone F10-89-4, red histogram). Lymphocytes gated blood from healthy volunteer with FL-7 FMO was used as control (blue histogram). The data were generated by using Beckman Coulter CytoFLEX flowcytometer and analyzed in FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147254 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated (FC00555-mFluor540)
Hello CJ!
FC00555-mFluor540 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis
Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis
Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated
Question
I was wanting to use your anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated for Flow Cytometry for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
As indicated on the product datasheet, FC00555-mFluor540 anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-04
Question
Is this FC00555-mFluor540 anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated reactive to the isotypes of PTPRC?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The immunogen of FC00555-mFluor540 anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated is Human T lymphocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-30
Question
Does anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 work for Flow Cytometry with erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
According to the expression profile of erythroleukemia, PTPRC is highly expressed in erythroleukemia. So, it is likely that anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 will work for Flow Cytometry with erythroleukemia.
Boster Scientific Support
Answered: 2020-02-14
Question
you antibody to test anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 on human erythroleukemia for research purposes, then I may be interested in using anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Huang
Verified customer
Asked: 2018-12-31
Answer
The products we sell, including anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-04
Question
I have attached the WB image, lot number and protocol we used for erythroleukemia using anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540. Please let me know if you require anything else.
A. Li
Verified customer
Asked: 2013-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-12
