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Product Info Summary
| SKU: | M01335-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-c-Kit Rabbit Monoclonal Antibody
View all CD117/c-kit Antibodies
SKU/Catalog Number
M01335-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-c-Kit Rabbit Monoclonal Antibody catalog # M01335-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Kit Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01335-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CGF-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Kit
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01335-1 is reactive to KIT in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
145 kDa
Calculated molecular weight
109865 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01335-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human HEL whole cell, human RT4 whole cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of c-Kit using anti-c-Kit antibody (M01335-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Kit antigen affinity purified monoclonal antibody (Catalog # M01335-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Kit at approximately 145 kDa. The expected band size for c-Kit is at 110 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using c-Kit Antibody .
Protein Target Info & Infographic
Gene/Protein Information For KIT (Source: Uniprot.org, NCBI)
Gene Name
KIT
Full Name
Mast/stem cell growth factor receptor Kit
Weight
109865 MW
Superfamily
protein kinase superfamily
Alternative Names
Mast/stem cell growth factor receptor Kit;SCFR;2.7.10.1;Piebald trait protein;PBT;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;p145 c-kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;CD117;KIT;SCFR; KIT C-Kit, CD117, MASTC, PBT, SCFR KIT proto-oncogene, receptor tyrosine kinase mast/stem cell growth factor receptor Kit|c-Kit protooncogene|p145 c-kit|piebald trait protein|proto-oncogene c-Kit|proto-oncogene tyrosine-protein kinase Kit|soluble KIT variant 1|tyrosine-protein kinase Kit|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIT, check out the KIT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Kit Rabbit Monoclonal Antibody (M01335-1)
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