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Product Info Summary
| SKU: | M00555-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Hu CD45 Purified PTPRC Monoclonal Antibody
SKU/Catalog Number
M00555-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Catalog# M00555-3). Tested in Flow Cytometry, IP, WB, IHC-P, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00555-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-28
Isotype
Mouse IgG1
Immunogen
Human thymocytes and T lymphocytes. The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00555-3 is reactive to PTPRC in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
147486 MW
Background of PTPRC
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00555-3 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used.
Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
Flow cytometry: Recommended dilution: 1 μg/ml.
Validation Images & Assay Conditions
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Figure 3. Flow Cytometry validation of PTPRC using Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3).
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (MEM-28) purified
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Figure 1. Immunocytochemistry staining of PTPRC using Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3).
Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28
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Figure 2. Immunohistochemistry validation of PTPRC using Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3).
Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28)Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147486 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
LCA, T200, LY5, B220, GP180, TPC PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3)
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