Anti-Hu CD243 Purified ABCB1 Monoclonal Antibody

Product Name

Anti-Hu CD243 Purified ABCB1 Monoclonal Antibody

View all MDR1/ABCB1 Antibodies

SKU/Catalog Number

M00049-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD243 Purified ABCB1 Monoclonal Antibody (Catalog# M00049-2). Tested in Flow Cytometry, IP, IHC-P, IHC-F application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD243 Purified ABCB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00049-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

UIC2

Isotype

Mouse IgG2a kappa

Immunogen

NIH 3T3 cells transfected with human CD243 (MDR-1) cDNA. The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00049-2 is reactive to ABCB1 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

141479 MW

Background of MDR1/ABCB1

CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC)-containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00049-2 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval in citrate buffer pH 6.
Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For ABCB1 (Source: Uniprot.org, NCBI)

Alternative Names

P-Glycoprotein, CLCS, MDR1, PGY1, ABC20, GP170, ABCB1, ABC transporter ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170 ATP binding cassette subfamily B member 1 ATP-dependent translocase ABCB1|ATP-binding cassette, sub-family B (MDR/TAP), member 1|P-glycoprotein 1|colchicin sensitivity|doxorubicin resistance|multidrug resistance protein 1|phospholipid transporter ABCB1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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