Product Info Summary
SKU: | M00458-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IP, IHC-P, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody
View all Integrin beta 2/CD18 Antibodies
SKU/Catalog Number
M00458-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody (Catalog# M00458-2). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00458-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-48
Isotype
Mouse IgG1
Immunogen
Leukocytes of a patient suffering from a LGL-type leukemia. The antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00458-2 is reactive to ITGB2 in Human
Applications
M00458-2 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
84782 MW
Background of Integrin beta 2/CD18
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions.
Flow cytometry: 2 μg/ml.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: spleen, microglia.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ITGB2 using Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody (M00458-2).
Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody M00458-2
Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48).Lane 1: KG-1a human acute myeloid leukemia cell line
Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ITGB2 (Source: Uniprot.org, NCBI)
Gene Name
ITGB2
Full Name
Integrin beta-2
Weight
84782 MW
Superfamily
integrin beta chain family
Alternative Names
959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta ITGB2 CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7 integrin subunit beta 2 integrin beta-2|cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor)|complement component 3 receptor 3 and 4 subunit|complement receptor C3 beta-subunit|integrin beta chain, beta 2|integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)|leukocyte cell adhesion molecule CD18|leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C|truncated integrin beta-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGB2, check out the ITGB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody (M00458-2)
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