Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody

Product Name

Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody

View all SIGLEC5 Antibodies

SKU/Catalog Number

M06510

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody (Catalog# M06510). Tested in Flow Cytometry, IP, IHC-F, ICC, ELISA application(s). This antibody reacts with Primate, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06510)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

1A5

Isotype

Mouse IgG1

Immunogen

Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1. The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M06510 is reactive to SIGLEC5 in Human, Primate

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

60715 MW

Background of SIGLEC5

CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06510 is guaranteed for ELISA, Flow Cytometry, IP, IHC-F, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.
Immunohistochemistry: 5-10 μg/ml.

Validation Images & Assay Conditions

Gene/Protein Information For SIGLEC5 (Source: Uniprot.org, NCBI)

Alternative Names

OBBP2, OB-BP2, SIGLEC-5, SIGLEC5 SIGLEC5 CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5 sialic acid binding Ig like lectin 5 sialic acid-binding Ig-like lectin 5|CD33 -like 2|OB-binding protein 2|obesity-binding protein 2|sialic acid-binding immunoglobulin-like lectin 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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