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Product Info Summary
| SKU: | M01114-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC |
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Product info
Product Name
Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody
View all CD40 Ligand/TNFSF5 Antibodies
SKU/Catalog Number
M01114-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (Catalog# M01114-2). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01114-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
24-31
Isotype
Mouse IgG1
Immunogen
Human CD154 fusion protein. The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01114-2 is reactive to CD40LG in Human, Primate
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
29370 MW
Background of CD40 Ligand/TNFSF5
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01114-2 is guaranteed for Flow Cytometry, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2-6 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of Cd40lg using Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (M01114-2).
Flow cytometry surface staining pattern of human peripheral blood mononuclear cells (PMA + ionomycin activated) stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 μg/ml) GAM APC.
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Figure 2. Flow Cytometry validation of Cd40lg using Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (M01114-2).
Separation of human CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood mononuclear cells (PMA + ionomycin activated) stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 μg/ml) GAM APC.Protein Target Info & Infographic
Gene/Protein Information For CD40LG (Source: Uniprot.org, NCBI)
Gene Name
CD40LG
Full Name
CD40 ligand
Weight
29370 MW
Superfamily
tumor necrosis factor family
Alternative Names
CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM CD40LG CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD40 ligand CD40 ligand|CD40 ligand|CD40-L|T-B cell-activating molecule|T-cell Gp39|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40LG, check out the CD40LG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40LG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (M01114-2)
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