Anti-Hu CD123 Purified IL3RA Monoclonal Antibody

Product Name

Anti-Hu CD123 Purified IL3RA Monoclonal Antibody

View all IL-3R alpha/CD123 Antibodies

SKU/Catalog Number

M04307

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD123 Purified IL3RA Monoclonal Antibody (Catalog# M04307). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD123 Purified IL3RA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04307)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

6H6

Isotype

Mouse IgG1

Immunogen

IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells. The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04307 is reactive to IL3RA in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

43330 MW

Background of IL-3R alpha/CD123

CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04307 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For IL3RA (Source: Uniprot.org, NCBI)

Alternative Names

IL3RA, MGC34174, hIL-3Ra IL3RA CD123, IL3RY, IL3RX, IL3RY, hIL-3Ra, IL3RA interleukin 3 receptor subunit alpha interleukin-3 receptor subunit alpha|CD123 |IL-3 receptor subunit alpha|IL-3R subunit alpha|IL-3R-alpha|IL-3RA|interleukin 3 receptor, alpha (low affinity)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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