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Product Info Summary
| SKU: | M01692 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody
View all HSP90 beta Antibodies
SKU/Catalog Number
M01692
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody catalog # M01692. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01692)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CCC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp90 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01692 is reactive to HSP90AB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
83264 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01692 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human Raji whole cell, human Jurkat whole cell, human HepG2 whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
IHC: human kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSP90AB1 using anti-HSP90AB1 antibody (M01692).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 antigen affinity purified monoclonal antibody (Catalog # M01692) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AB1 at approximately 90 kDa. The expected band size for HSP90AB1 is at 83 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Hsp90 beta antibody .
Protein Target Info & Infographic
Gene/Protein Information For HSP90AB1 (Source: Uniprot.org, NCBI)
Gene Name
HSP90AB1
Full Name
Heat shock protein HSP 90-beta
Weight
83264 MW
Superfamily
heat shock protein 90 family
Alternative Names
Heat shock protein HSP 90-beta;HSP 90;Heat shock 84 kDa;HSP 84;HSP84;HSP90AB1;HSP90B, HSPC2, HSPCB; HSP90AB1 D6S182, HSP84, HSP90B, HSPC2, HSPCB heat shock protein 90 alpha family class B member 1 heat shock protein HSP 90-beta|HSP90-beta|heat shock 84 kDa|heat shock 90kD protein 1, beta|heat shock protein 90 kDa|heat shock protein 90kDa alpha (cytosolic), class B member 1|heat shock protein 90kDa alpha family class B member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSP90AB1, check out the HSP90AB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSP90AB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp90 beta HSP90AB1 Rabbit Monoclonal Antibody (M01692)
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