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Product Info Summary
| SKU: | A01692S254 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-HSP90B (phospho-S254) HSP90AB1 Antibody
View all HSP90 beta Antibodies
SKU/Catalog Number
A01692S254
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HSP90B (phospho-S254) HSP90AB1 Antibody catalog # A01692S254. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP90B (phospho-S254) HSP90AB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01692S254)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human HSP90B around the phosphorylation site of Serine 254.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01692S254 is reactive to HSP90AB1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
83264 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01692S254 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:5000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of HSP90AB1 using Anti-HSP90B (phospho-S254) HSP90AB1 Antibody (A01692S254).
Immunohistochemistry (IHC) analyzes of HSP90B (phospho-S254) polyclonal antibody in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-HSP90B (phospho-S254) HSP90AB1 Antibody A01692S254
Western blot (WB) analysis of HSP90B (phospho-S254) pAb at 1:5000 dilutionLane1:MCF-7 whole cell lysate(40ug)
Lane2:The Testis tissue lysate of Mouse(40ug)
Lane3:The Testis tissue lysate of Rat(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Lane5:Hela whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For HSP90AB1 (Source: Uniprot.org, NCBI)
Gene Name
HSP90AB1
Full Name
Heat shock protein HSP 90-beta
Weight
83264 MW
Superfamily
heat shock protein 90 family
Alternative Names
Heat shock protein HSP 90-beta;HSP 90;Heat shock 84 kDa;HSP 84;HSP84;HSP90AB1;HSP90B, HSPC2, HSPCB; HSP90AB1 D6S182, HSP84, HSP90B, HSPC2, HSPCB heat shock protein 90 alpha family class B member 1 heat shock protein HSP 90-beta|HSP90-beta|heat shock 84 kDa|heat shock 90kD protein 1, beta|heat shock protein 90 kDa|heat shock protein 90kDa alpha (cytosolic), class B member 1|heat shock protein 90kDa alpha family class B member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSP90AB1, check out the HSP90AB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSP90AB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP90B (phospho-S254) HSP90AB1 Antibody (A01692S254)
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